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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
NB.21
L × i.d.:
2 cm × 4 mm
Particle size:
5 μm
Matrix active group:
C18 (octadecyl) phase
Pore size:
120 Å
Matrix:
silica gel high purity, spherical
Product Name
SUPELCOSIL™ LC-18 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
agency
suitable for USP L1
packaging
pkg of 1 kit
parameter
<70 °C temp. range
technique(s)
HPLC: suitable
L × I.D.
2 cm × 4 mm
matrix
silica gel high purity, spherical
matrix active group
C18 (octadecyl) phase
particle size
5 μm
pore size
120 Å
pH range
2-7.5
application(s)
food and beverages
compatibility
use to protect LC-18, LC-PAH
separation technique
reversed phase
Quality Level
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Application
SUPELCOSIL™ LC-18 Supelguard Cartridge may be used as a guard column along with SUPELCOSIL™ LC-18 HPLC Column during the analysis of:
- Synthetic food dyes in commercial soft drinks by ion-pair high performance liquid chromatography (HPLC IP).
- Drugs in plasma and brain samples by solid-phase extraction (SPE) and protein precipitation in conjunction with HPLC combined with ultraviolet (UV) detection.
Other Notes
Kit includes one cartridge, a stand-alone holder, a piece of tubing, and 2 nuts and ferrules.
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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Practice of solid-phase extraction and protein precipitation in the 96-well format combined with high-performance liquid chromatography-ultraviolet detection for the analysis of drugs in plasma and brain.
Rouan MC, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 754(1), 45-55 (2001)
Determination of synthetic food dyes in commercial soft drinks by TLC and ion-pair HPLC.
de Andrade FI, et al.
Food Chemistry, 157, 193-198 (2014)
K M Matar et al.
Therapeutic drug monitoring, 21(5), 559-566 (1999-10-16)
A simple, rapid, sensitive, and reproducible high-performance liquid chromatographic (HPLC) method for simultaneous determination of the antiepileptic drugs (ethosuximide, primidone, lamotrigine, phenobarbital, phenytoin, and carbamazepine) and two metabolites (carbamazepine-diol and carbamazepine-epoxide) in human plasma is described. The procedure involves extraction
V Stocchi et al.
Analytical biochemistry, 167(1), 181-190 (1987-11-15)
A simple and fast ion pair reversed-phase high-performance liquid chromatographic method has been developed for the simultaneous determination of ATP, ADP, AMP, GTP, GDP, IMP, NADP+, NADPH+, NAD+, NADH, ADP-ribose, inosine, adenosine, hypoxanthine, and xanthine. This method allows us to
G R Granneman et al.
Clinical pharmacokinetics, 29 Suppl 2, 1-8 (1995-01-01)
A rapid and sensitive assay was developed for the measurement of plasma concentrations of zileuton racemate, a potent inhibitor of 5-lipoxygenase. Zileuton and its inactive N-dehydroxylated metabolite were extracted from human, monkey, and rat plasma by use of a solid-phase
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