Skip to Content
Merck
CN

Key Documents

View All Documentation

67463-U

BIOshell™ A400 Protein C18 (3.4 μm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
L × i.d.:
10 cm × 2.1 mm
Particle size:
3.4 μm
Matrix active group:
C18 (octadecyl) phase
Pore size:
3.4 μm particle size, 400 Å
Matrix:
spherical silica particle platform, superficially porous particle
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

BIOshell™ A400 Protein C18 Column, L × I.D. 10 cm × 2.1 mm, 3.4 μm particle size

material

stainless steel hardware (column)

Quality Level

100

agency

suitable for USP L1

description

Shell Thickness (0.2 μm), Solid Core (3.0 μm)

product line

BIOshell™

feature

endcapped

analyte chemical class(es)

proteins

manufacturer/tradename

BIOshell™

packaging

1 ea of

extent of labeling

1% carbon loading

parameter

600 bar max. pressure, 90 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

10 cm × 2.1 mm

surface area

~15 m2/g

matrix

spherical silica particle platform, superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

3.4 μm

pore size

3.4 μm particle size, 400 Å

operating pH

1-8

separation technique

reversed phase

General description

BIOshell™ 400 Å Protein C18 columns contain 3.4 μm advanced Fused-Core® particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns offer maximum hydrophobicity for resolving tricky hydrophilic and PEGylated proteins. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C18 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 1 to 9.

Application

UHPLC Analysis of Proteins on BIOshell™ A160 Peptide C18 2.7 μm; BIOshell™ A400 Protein C18, 3.4 μm; and BIOshell™ IgG 1000 Å C18, 2.7 μm

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Still not finding the right product?

Explore all of our products under BIOshell A400 Protein C18 Column

Regulatory Information

新产品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
USPWH001274
USPWH001273
USPWH001272
USPWH001271
USPWH001268

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Analysis of mAb and ADC on Widepore Silica Monolith Column

Intact analysis of Universal Antibody Standard human and Antibody-Drug Conjugate (ADC) Mimic on silica monolithic HPLC columns under various conditions.

Global Trade Item Number

SKUGTIN
67463-U04061833364994