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Merck
CN

67469-U

BIOshell™ A400 Protein C18 (3.4 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

Synonym(s):

Superficially Porous Wide Pore C18 UHPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

Key Documents

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Product Name

BIOshell™ A400 Protein C18 Column, L × I.D. 15 cm × 2.1 mm, 3.4 μm particle size

material

stainless steel hardware (column)

Quality Level

100

Agency

suitable for USP L1

description

Shell Thickness (0.2 μm)
Solid Core (3.0 μm)

product line

BIOshell™

feature

endcapped

analyte chemical class(es)

proteins

manufacturer/tradename

BIOshell™

packaging

1 ea of

extent of labeling

1% carbon loading

parameter

600 bar max. pressure
90 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

15 cm × 2.1 mm

surface area

~15 m2/g

matrix

spherical silica particle platform
superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

3.4 μm

pore size

3.4 μm particle size
400 Å

operating pH

1-8

separation technique

reversed phase

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General description

BIOshell™ 400 Å Protein C18 columns contain 3.4 μm advanced Fused-Core® particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns offer maximum hydrophobicity for resolving tricky hydrophilic and PEGylated proteins. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C18 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 1 to 9.

Application

UHPLC Analysis of Proteins on BIOshell™ A160 Peptide C18 2.7 μm; BIOshell™ A400 Protein C18, 3.4 μm; and BIOshell™ IgG 1000 Å C18, 2.7 μm

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Certificates of Analysis (COA)

Lot/Batch Number
USGEQ001575
USGEQ001574
USGEQ001569
USGEQ001568
USGEQ001566

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Care and Use Sheet for BIOshell A400 Protein C18 Column

Care & Use Sheet for BIOshell™ A400 Protein C18 Column

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