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BIOshell^™ A400 Protein C18 (3.4 μm) HPLC Columns

Name: BIOshell<SUP>&#8482;</SUP> A400 Protein C18 (3.4 μm) HPLC Columns
Brand: SUPELCO

L × I.D. 5 cm × 300 μm, HPLC Column

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About This Item

UNSPSC Code:

41115700

NACRES:

SB.52

L × i.d.:

5 cm × 300 μm

Particle size:

3.4 μm

Matrix active group:

C18 (octadecyl) phase

Pore size:

3.4 μm particle size, 400 Å

Matrix:

spherical silica particle platform, superficially porous particle

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Properties

Product Name

BIOshell^™ A400 Protein C18 Column, L × I.D. 5 cm × 300 μm, 3.4 μm particle size

material

stainless steel hardware (column)

Quality Segment

100

agency

suitable for USP L1

description

Shell Thickness (0.2 μm), Solid Core (3.0 μm)

product line

BIOshell^™

mol wt

2000 - 500,000

feature

endcapped

analyte chemical class(es)

proteins

manufacturer/tradename

BIOshell^™

packaging

1 ea of

extent of labeling

1% carbon loading

parameter

600 bar max. pressure, 90 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

5 cm × 300 μm

surface area

~15 m²/g

matrix

spherical silica particle platform, superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

3.4 μm

pore size

3.4 μm particle size, 400 Å

operating pH

1-8

separation technique

reversed phase

Description

General description

BIOshell^™ 400 Å Protein C18 columns contain 3.4 μm advanced Fused-Core^® particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns offer maximum hydrophobicity for resolving tricky hydrophilic and PEGylated proteins. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C18 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 1 to 9.

Application

UHPLC Analysis of Proteins on BIOshell^™ A160 Peptide C18 2.7 μm; BIOshell^™ A400 Protein C18, 3.4 μm; and BIOshell^™ IgG 1000 Å C18, 2.7 μm

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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