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TOYOPEARL® Butyl-650M Bulk Media

matrix polymer (65 μm), bottle of 100 mL, 65 μm

Synonym(s):

TOYOPEARL® Butyl-650 HIC Bulk Media

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About This Item

UNSPSC Code:
41115700
NACRES:
NB.21
Particle size:
65 μm
Matrix active group:
C4 phase
Matrix:
polymer (65 μm)
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description

Butyl-650M

product line

TOYOPEARL®

form

slurry

mol wt

2000 - 1,000,000

packaging

bottle of 100 mL

parameter

3 bar max. pressure

technique(s)

HPLC: suitable

matrix

polymer (65 μm)

matrix active group

C4 phase

particle size

65 μm

operating pH

1-13

capacity

40 g/L±10 adsorption capacity (lysozyme)

separation technique

hydrophobic interaction (HIC)

General description

TOYOPEARL® HIC resins are hydrophobic interaction chromatography resins that offer the following advantages: strong affinity for water-soluble proteins, high recovery of mass and activity, high sample capacity up to 2-4 times that of gel media, fluctuating salt concentrations will not change the bed volume, mechanical stability to 7kg/cm squared (7 bar/100psi), stability at a wide pH range (2-12), clean in place with 0.5M NaOH, and autoclavable. The exclusion limit of 5x106 Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects.
TOYOPEARL® HIC resins for hydrophobic interaction chromatography offer these advantages:
  • Strong affinity for water-soluble proteins
  • High recovery of mass and activity
  • High sample capacity: up to 2-4 times that of gel media
  • Fluctuating salt concentrations will not change bed volume
  • Mechanical stability to 7kg/cm2 (7 bar/100psi)
  • Stable at wide pH range: 2-12
  • Clean in place with 0.5M NaOH
  • Autoclavable
     
TOYOPEARL media offer high yield recovery of proteins, using various aqueous eluants. The exclusion limit of 5x106 Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects. Packed as 100mL bottle.

Application

TOYOPEARL® media are used in hydrophobic interaction media, resins and separation media. TOYOPEARL® media offer high yield recovery of proteins, using various aqueous eluants.

Features and Benefits

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physical form

Shipped in 20% (v/v) ethanol.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Toyopearl Butyl-650 resin has the second highest hydrophobicity of the HIC ligands offered by TBL. Its pore size is the largest of our three butyl resins. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in interresinte purification and polishing steps

Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

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Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Codes

H226

Hazard Classifications

Flam. Liq. 3

WGK

WGK 1

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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