Supelco® HPLC and UHPLC columns meet today’s demands of fast U/HPLC, LC-MS, and biopolymer separation, as well as regulated pharmacopeia and agency methods within various industries. Supelco® HPLC products are available with Fused-Core® particle technology, monolithic silica, and fully porous particles, including ultra-pure silica. In addition, porous graphitic carbon (PGC) particles, and polymeric particles are available for high pH stability.
The comprehensive Supelco® portfolio of analytical solutions to meet your U/HPLC and LC-MS needs.
High performance liquid chromatography (HPLC) is a technique for separating analytes dissolved in a liquid, mobile phase by using their specific interaction with a stationary phase (chromatography column).
Based on Fused-Core® particle technology, Ascentis® Express columns provide a exceptional advancements in HPLC column performance and the benefits of high sample throughput at maximum resolution.
The BIOshell™ line of superficially porous particle (SPP) packed columns has been developed to aid research into understanding the subtleties of the molecule that is being developed.
To truly accelerate chromatographic separations, there is no better choice than Chromolith® HPLC columns. Due to their revolutionary, monolithic technology, Chromolith® columns provide excellent and rapid separations with extremely high robustness and matrix tolerance compared to particulate columns.
The success of any method depends on the quality of the stationary phase. Precise, long-term reproducibility is a key factor in achieving reliable results. The base silica of Purospher™ STAR columns is 99.999% pure.
Columns for versatile and robust Chiral HPLC and LC-MS separations.
The use of HPLC systems to achieve faster analyses and increased resolution pushes systems toward higher operating pressures. For instance, faster flow rates, longer column lengths, and reduced particle sizes gain a performance advantage in HPLC, but at a cost of increased operating pressures.
Many of the challenges facing researchers in the proteomics and biopharmaceutical fields are related to the need to obtain as much information as possible on very limited samples. We designed Discovery® BIO Wide Pore reversed-phase HPLC columns to address these challenges.
In this guide, we provide a premier selection of proven Supelco® tools and consumables that meet the requirements of scientists who primarily use the LC-MS technique for analysis of drugs, biomolecules separation and analytical assays.
In this technical bulletin, you will learn some tips on identifying LC-MS contaminants and avoiding contamination. Our 110 years of separation expertise, combined with our precision-manufactured products, give you the greatest chance of obtaining reproducible, clean data.
Since the 1970’s, Supelco® has been a trusted name in high performance liquid chromatography (HPLC) consumables. From a diverse array of columns packed with different stationary phases to a complete portfolio of HPLC accessories, the Supelco® portfolio of HPLC consumables caters to the needs of all analysts.
Application compilation with focus on the LC-MS analysis of drugs of abuse in biological samples.
Easily transfer a method between HPLC columns, (scale between microbore through preparative range), based on the 2 column dimensions and current method conditions.
The official pharmaceutical analysis monographs in the United States Pharmacopeia (USP) detail the methods used by pharmaceutical manufacturers for quality control of bulk drug substances and dosage form preparations.
To maximize the lifetime of an HPLC column and ensure its top performance, it is necessary to properly maintain the column. Many different factors can contribute to loss of resolution or sensitivity, but often times the following factors lead to the vast majority of column performance issues.
Porous graphitic carbon (PGC) columns, like the Supel™ Carbon LC column, provide unique chromatographic properties that are unmatched by silica particle-packed columns. The full pH range can be used as well as high temperature (> 200 °C), opening up new frontiers for chromatographers.
Superficially porous silica columns deliver higher speed and efficiency than fully porous particles of the same size. Fused-Core® HPLC columns like Ascentis® Express and BIOshell™ can convert any HPLC system into a fast HPLC workhorse with maximum speed and performance.
We have a wide range of chiral HPLC columns for various applications.
In this webcast, the origins and problems in LC–MS arising from impurities and contaminations will be described in detail and tips and tricks will be provided in order to enable or maintain high quality and high sensitivity LC-MS analyses.
Learn about porous graphitic carbon, a stationary phase material that is fully porous, carbon-based material—it is capable of analyzing small molecules and smaller proteins and peptides.
Quantitation of monoclonal antibodies by LC-MS is a difficult analytical challenge due to the complexity in the workflow, from sample prep to LC-MS quantitation. This presentation will highlight a simple and high throughput method for the quantitation of monoclonal antibodies and proteins in human and animal serum.
The focus of this seminar is to provide an overview of different strategies and methods employed over the past several years in characterizing samples containing chiral compounds.
This presentation focuses on recent advances, in the past two years, in 'carbon-HPLC' in the analysis of compounds classically associated with being difficult to separate by conventional 'silica-HPLC.'
Join our webinar focusing on "How to Increase The Cost Effectiveness for HPLC Separations of Challenging and Matrix-rich Samples" for the potential for cost and time savings by increasing lab productivity with faster separations and column materials providing very high matrix tolerance.
*Tosoh Bioscience columns are available in the following countries:
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