Millicell® cell culture inserts are designed to maximize biological relevance and user convenience in cell culture applications. In vivo, cells live in a three-dimensional environment and can access nutrients from all directions. In contrast, traditional plastic culture plates force cells to grow on a smooth, two-dimensional surface, leading to flattened nuclei and poor function. Millicell® permeable inserts and plates feature a design that allows access to both the basolateral and apical surfaces of cell layers. This promotes natural, multidimensional growth and provides more relevant in vitro models, leading to more biologically relevant results for applications including toxicity screening, primary and secondary screening, cell signaling, cell proliferation, transport assays, and ADME/Tox drug safety studies.
Cell culture with Millicell® hanging inserts
Millicell® hanging inserts make it possible to study both apical and basal surfaces of the cell monolayer, and are excellent tools for co-culture, as there is no disturbance of the outer basolateral cell culture by the presence of insert feet.
Millicell® Standing Inserts are free-standing inserts that can rest at the bottom of cell culture plate wells and other vessels, elevated by insert feet. These standing inserts promote the natural growth of suspension and adherent cell lines and are available in three different optimized membranes tailored for specific needs.
Millicell® Organotypic Standing Inserts are designed for superior study of three-dimensional explant structures.
Millicell®-24 and Millicell®-96 Plate Assemblies enable cell growth, feeding, and analysis in one membrane-bottom plate optimized to grow and sustain high-integrity cell monolayers. These plates can be used manually or with automated cell seeding, feeding, and washing systems. Their thoughtful design improves function with seed-and-feed systems, automated liquid handling systems, and transepithelial electrical resistance (TEER) measurement instruments.
Millicell® ULA plates are reliable 3D cell culture tools that promote scaffold free self-assembly of spheroids. The 96-well U bottom plates are compatible with analysis and brightfield and fluorescence imaging as the wells have high optical clarity. The non-cytotoxic, stable, and non-adhesion surface of Millicell® ULA plates support high throughput spheroid assembly.
Millicell® Microwell plates are ready-to-use 3D culture tools for high throughput and reproducible organoid culture. Based on an array of ultra-dense U-bottom shaped microwells (μW), single organoids are generated in each microcavity and grown in a pre-cast hydrogel without a solid extracellular matrix. With multiple configurations and microwell diameters available, Millicell® Microwell plates fit your organoid application needs.
Recommended membrane material and pore sizes for Millicell® cell culture inserts and plates are based on cell type and application.
Millicell® EZ Slides can be used to culture, fix, stain and view your cells all in one device. There’s no need to tediously move cover slips from your culture dish to a slide, or to remove the media chamber from the slide prior to fixing or staining. Easily remove the chamber structure without worrying about breaking slides or ruining cell layers. Acquire data simply and quickly with Millicell® EZ Slides.
Millicell® HY Flasks are innovative multilayer cell culture flasks that support uniform, consistent cell growth for high cell recovery. Manufactured with multiple identical layers, these flasks maximize cell culture surface area for the highest yields, while saving incubator space.
Solutions for high-yield cell culture scale-up applications including multi-layered flasks (HyperFlasks/CellStacks), roller bottles, spinner and shaker flasks. Free ECACC handbook download.
This guide for pharmaceutical development, discovery research, molecular biology and sample preparation includes products for screening and cell culture. These products provide proven solutions for a range of applications and are backed by extensive technical support.
The research needed to narrow the gap between in vitro and in vivo, or 2D and 3D, cell environments has never been greater. Our sixty-plus year legacy of precision engineered filtration and cell culture expertise continues to provide the modern tools needed to support cell growth that is more predictive of natural cellular behavior.
Millicell® cell culture inserts and plates provide a permeable support for cell growth, allowing cells access to media and nutrients from the apical and basolateral sides. This feature more closely mimics the in vivo cellular environment of cells compared to conventional seeding on polystyrene plates.
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.
How to set up cell migration assays using cell culture inserts.
16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.
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