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Mammalian Cell Lines

Fluorescent staining of cultured mammalian cells

We are partnered with the European Collection of Authenticated Cell Cultures (ECACC) repository, part of Public Health England, to offer quality-controlled cell lines and nucleic acids to laboratories around the world. Browse our portfolio of authenticated, validated, mycoplasma-free cell lines for biomedical research, protein production, hybridoma fusion, virus propagation, and more—including hundreds of cancer cell lines, and iPS cells from the European Bank of induced Pluripotent Stem Cells (EBiSC). Cell line product pages also include ordering information for DNA, RNA and cDNA derived from corresponding cells, which allow researchers to screen for genes or gene expression patterns and identify the cell lines most suitable for their research.

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Product Number
Product Name
Product Description
Pricing
SCC066
Blood-Brain Barrier hCMEC/D3 Cell Line
The hCMEC/D3 BBB cell line has been extensively characterized for brain endothelial phenotype and is a model of human blood-brain barrier (BBB) function.
SCC109
AC16 Human Cardiomyocyte Cell Line
AC16 Human Cardiomyocytes can be serially passaged and can differentiate when cultured in mitogen-free medium. The cells may be used to study developmental regulation of cardiomyocytes.
SCC065
HL-1 Cardiac Muscle Cell Line
HL-1 Cardiac Muscle Cell Line has been extensively characterized and is a valuable model system to address questions of cardiac biology at the cellular & molecular levels.
SCC064
LX-2 Human Hepatic Stellate Cell Line
The LX-2 human hepatic stellate cell line has been extensively characterized and retain key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them a highly suitable model of human hepatic fibrosis.
SCC129
HT-22 Mouse Hippocampal Neuronal Cell Line
HT-22 mouse neuronal cell line is a valuable cell model for studies of glutamate-induced toxicity in neuronal cells.
SCC150
16HBE14o- Human Bronchial Epithelial Cell Line
16HBE14o- human bronchial epithelial cell line is widely used to model barrier function of the airway epithelium and to study respiratory ion transport as well as the function of CFTR.
SCC148
MB49 Mouse Bladder Carcinoma Cell Line
MB49 mouse urothelial carcinoma cell line is widely used as an in vitro and in vivo model of bladder cancer.
SCC145
ID8 Mouse Ovarian Surface Epithelial Cell Line
ID8 mouse ovarian surface epithelial cell line is frequently used as a syngeneic mouse model for human ovarian cancer.
SCC194
CT-2A Mouse Glioma Cell Line
CT-2A mouse glioma cell line is a valuable mouse model for therapeutic research on brain malignancies.
SCC062
HMC-1.2 Human Mast Cell Line
HMC-1.2 human mast cell line is a variant subline of the HMC-1 cell line and possesses both the V560G and D816V KIT mutations.
SCC208
INS-1 832/3 Rat Insulinoma Cell Line
INS-1 832/3 rat insulinoma cell line is a useful model for insulin secretion regulation and pancreatic islet beta-cell function studies.
SCC067
HMC-1.1 Human Mast Cell Line
HMC-1.1 human mast cell line is a variant subline of the HMC-1 cell line and possesses the V560G KIT mutation.
SCC111
R-Spondin1 expressing 293T Cell Line
The R-Spondin1-Expressing 293T cell line produces RSPO1, a critical protein used to establish 3D organoid cultures from stomach, small intestine, colon, pancreas, and liver sources.
SCC043
C28/I2 Human Chondrocyte Cell Line
C28/I2 Human Chondrocyte Cell Line is widely used as a model cell line for studying normal and pathological cartilage repair mechanisms related to chondrocyte biology and physiology.
SCC041
C20A4 Human Chondrocyte Cell Line
C20A4 Human Chondrocyte Cell Line is widely used as a model cell line for studying normal and pathological cartilage repair mechanisms related to chondrocyte biology and physiology.
SCC163
HOG Human Oligodendroglioma Cell Line
HOG human oligodendroglioma cell line is a useful model to study the role of oligodendrocytes in neurodegenerative diseases.
SCC147
TPC-1 Human Papillary Thyroid Carcinoma Cell line
TPC-1 human papillary thyroid carcinoma cell line is a highly characterized model for thyroid cancer research.
SCC173
MCA205 Mouse Fibrosarcoma Cell Line
MCA205 mouse fibrosarcoma cell line is an excellent model for studying immune response to tumor cells and the development of targeted cancer immunotherapies.
SCC178
AT-3 Mouse Mammary Carcinoma Cell Line
AT-3 mouse mammary tumor cell line may be used to develop mouse models for mammary cancer studies.
SCC207
INS-1 832/13 Rat Insulinoma Cell Line
INS-1 832/13 rat insulinoma cell line is a useful model for insulin secretion regulation and pancreatic islet beta-cell function studies.
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Cell Lines

Our comprehensive offering of cells available with easy ordering and fast delivery also includes unique primate cell lines collected in accordance with guidelines established by the Convention on International Trade in Endangered Species (CITES), as well as the Human Leukocyte Antigen (HLA) typed collection, and hybridoma cell lines that produce monoclonal antibody against specific epitopes.

Our encyclopedic collection of cells includes lines from the following tissue types and species:

Cancer Cell Lines

Fluorescent staining of UFH-001 Human Breast Cancer Cells

Search cell lines by cancer type:

To ensure reliable propagation and reproducible results, we recommend using authenticated cell lines from a reputable source.  The Sigma-Aldrich® brand guarantees unsurpassed quality control, so the cell lines we offer are pathogen-free and identity-certified. Browse our collection of over 600 cancer cell lines.

Other Mammalian Cell Line Collections

Our extensive collection of cell lines goes beyond tissue-specific human and cancer cell lines.

  • Non-human primate cells in cooperation with CITES: cells collected in cooperation with The Convention on the International Trade in Endangered Species of Wild Fauna and Flora
  • HLA Collection: Human Leukocyte Antigen (HLA)-Typed Collection maintained by the European Collection of Cell Cultures (ECACC)
  • Hybridoma collection: cell lines optimized for the production of immunoglobulin and used in applications such as monoclonal antibody production
  • EBiSC iPS cell lines: inducible pluripotent stem cells from the European Bank
  • USCF Lung Epithelial cell lines: these cells are widely used to model barrier function of the airway epithelium and to study respiratory ion transport as well as the function of CFTR. These unique cell lines have also been used to study viral attachment and replication in SARS-CoV-2 infection.

Applications for Mammalian Cell Lines

Cell lines are used not only to model biological systems and disease, but for practical biotechnology uses in the production of proteins, viruses, and more. Explore cells used in these applications:

Related Product Literature
Thawing of Frozen Cell Lines
Cell culture protocol for proper thawing of cryopreserved cell lines.
Cryopreservation of Cell Lines
Cell culture protocol for freezing cell lines at high cell viabilities using cryopreservation reagents such as DMSO. Cryopreservation is a method whereby cells are frozen, maintaining their viability, until they are defrosted months or years later. Free ECACC handbook download.
ECACC Handbook
Fundamental Techniques in Cell Culture Laboratory Handbook – 4th Edition
Related Product Categories
Classical Media & Buffers
Cell culture media formulations have been optimized over decades of research in response to diverse cellular physiology. Select media such as DMEM, MEM, RPMI-1640, Ham’s F-10 & F-12 formulations, as well as serum-free solutions such as M199.
Supplements for Cell Culture
Cell culture supplements enable the customization of media with defined characteristics to support specific applications and serum-free culture needs. Our culture-grade supplements include ITS formulations, amino acids, vitamins, antibiotics, and reagents qualified for hybridoma and insect cell culture.
Fetal Bovine Serum (FBS)
FBS, or fetal bovine serum (also called FCS) is one of the most important supplements for mammalian cell culture. Choose FBS grade based on documentation requirements and application specificity.
Primary Cells
Primary cells are derived directly from living tissue and closely replicate the physiology of biological systems. Our collection includes blood, patient tumor xenografts, and hepatocytes essential for drug screening and ADME/Tox studies.
Stem Cells
Our complete range of ES and iPS cell-qualified products includes stem cell lines and media for differentiation and maturation, and provides stem cell researchers with convenient and cost-effective solutions for the reliable culture of pluripotent stem cells.
Stericup® Vacuum Filters
Essential Stericup® and sustainable Stericup® E filters are integral devices for filtering media to protect cell cultures. Choose from membranes and pore sizes for fast flow, low protein binding, mycoplasma exclusion, and plastic waste reduction.
Millipore® Sterile Filters
Prepare cell culture media and filter-sterilize aqueous solutions from a few mL to 20 L using industry-leading Millipore® sterile filtration devices. Choose fit-for-application membranes and pore sizes in Steriflip®, Sterivex®, Stericap®, and Steripak® devices.
Agarose: Properties and Research Applications
We offer agarose bioreagents in a selection of grades suitable for cell culture and molecular biology applications ranging from chromatography to nucleic acid & protein gel electrophoreses. The pHast Pack™ ready-to-pour agarose gels are formulated to save time and effort in native RNA and DNA electrophoresis assays.
Related Applications
Mammalian Cell Culture
Applications, principles, and methods for in vitro mammalian cell culture including links to articles, protocols, and videos for proper aseptic technique, passaging and subculturing, and culture media considerations.
Stem Cell Culture
An overview of different stem cell types, stem cell culture basics and stem cell applications in basic research and clinical research applications, such as tissue regeneration, genetic diseases and cancer.
Primary Cell Culture
Primary cells isolated directly from human and animal sources maintain physiological relevance of the tissues from which they are derived, and are used for ADME/Tox screening. Find applications and methods for culturing mammalian primary cells in vitro.
3D Cell Culture
Applications, principles, and techniques for three-dimensional (3D) cell culture. Includes techniques and descriptions of scaffold and scaffold-free methods matrices for the culture of complex in vitro models including organoids and spheroids.
Cell Culture Media Preparation
Culture media preparation for in vitro cell cultivation including preparation of different media formats, sterilization methods, cell growth and regulation supplements, media storage and use.
Cell Growth & Maintenance
A synopsis of the conditions, media, nutrients, cultureware, and environments required for successful propagation of mammalian and yeast cells. Nutrient reagents, sterile technique and filtration, and requirements for 2D, 3D, and suspension cultures are described.
Cell Culture Troubleshooting
Expert tips for correcting common problems with cell culture including bacterial and fungal contamination, debris in culture media, cell detachment or clumping, slow growth, and low viability.
Transfection & Gene Editing
Cell transfection principles, common transfection methods, and the role of transfection in gene editing and silencing for research, drug discovery, recombinant protein production.

Highlights

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