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Transfection

What is transfection? Transfection is the process of introducing genetic material, including DNA, RNA, and proteins, into eukaryotic cells for gene expression, gene knockdown, and many other applications. Transfection uses various physical, chemical, or biological methods and reagents to facilitate the transfer of the foreign material. Comparatively, bacterial transformation and viral transfection (viral transduction) are similar techniques that are suitable for transferring materials into different cell types for diverse downstream applications.  

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Product Number
Product Name
Product Description
Pricing
D8418
Dimethyl sulfoxide
for molecular biology
XTGHP-RO
X-tremeGENE™ HP DNA Transfection Reagent
High-performance polymer reagent for transfecting many cell lines
TR-1003
Polybrene Infection / Transfection Reagent
A highly efficient method of gene transfer into mammalian cells leveraging infection with retroviral vectors.
D9542
DAPI
for nucleic acid staining
XTG9-RO
X-tremeGENE™ 9 DNA Transfection Reagent
Polymer reagent for transfecting common cell lines
MBD0015
DAPI ready made solution
For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL
70967
GeneJuice® Transfection Reagent
Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammalian cells.
XTG360-RO
X-tremeGENE™ 360 Transfection Reagent
Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines
SITRAN-RO
X-tremeGENE™ siRNA Transfection Reagent
Polymer reagent for delivering siRNA to common cell lines
11202375001
DOTAP Liposomal Transfection Reagent
>99% (TLC), liquid, suitable for transfection
MBD0020
DAPI ready made solution with Antifade
For Nuclear counterstain in Fluorescence Microscopy
L3287
Escort™ IV Transfection Reagent
Lipid reagent for transient and stable transfection of mammalian and insect cells.
CAPHOS
Calcium Phosphate Transfection Kit
Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells
L3037
Escort™ III Transfection Reagent
Lipid reagent for transfecting sensitive and primary cells
71281
ProteoJuice Protein Transfection Reagent
Efficient protein delivery into mammalian cells
NPT01
NeuroPorter™ Transfection Kit
Lipid formulation for nucleic acid transfections in neuronal and glial cells
71259
Insect GeneJuice® Transfection Reagent
Proprietary liposome transfection reagent optimized for maximal transfection efficiency of Sf9 insect cells for baculovirus protein expression.
72181
293-Free Transfection Reagent
Animal-free polycationic liposomal transfection reagent optimized for the transfection of HEK293 cells grown in suspension culture.
50553
α-Tri-Calcium phosphate
Reagent for transient & stable DNA transfections
71115
RiboJuice siRNA Transfection Reagent
Efficient delivery of siRNA for targeted gene suppression
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Related Product Resources

Brochure: Genomics
Flyer: X-tremeGENE™ 360 Transfection Reagent
X-tremeGENE™ Transfection Reagents Comparison Guide

Calcium Phosphate, Lipid and Polymer-based Transfection Reagents for Delivering DNA, siRNA, miRNA, and CRISPR/Cas Components

The introduction of plasmid DNA or RNA into mammalian and insect cells has become routine in research labs. High-efficiency transfection in nearly any cell type can be achieved thanks to the wide variety of reagents that are currently available. We cover a variety of popular transfection reagents, including our Escort™ lipid reagents, NeuroPorter™ neuronal-specific reagent, X-tremeGENE™, and Genejuice transfection reagents. For help selecting the right reagent for your cells, explore our wide selection of transfection reagents within the product selection table or our Molecular Cloning and Protein Expression resource page for related products. 

Transfection reagents for DNA, siRNA, miRNA and CRISPR/RNP

X-tremeGENE™ Roche Transfection Reagents

With a joint commitment to enable your next discovery, we exclusively offer the complete portfolio of Roche X-tremeGENE™ transfection reagents. Roche, X-tremeGENE™, transfection reagents provide efficient delivery of DNA, siRNA, miRNA, and CRISPR/Cas9 components for common and difficult-to-transfect cell lines. The X-tremeGENE™ products are easy to optimize for your application needs and produce minimal toxicity.

Explore the X-tremeGENE™ Transfection Reagents General Recommendations guide to compare products.

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