What is protein gel electrophoresis? Protein gel electrophoresis is a method that often uses various systems and transfer equipment to separate a diverse mixture of proteins by their molecular weight. Subsequent protein analyses include protein purification, proteomics, and Western Blotting applications. A variety of electrophoresis cells and transfer systems for polyacrylamide gel electrophoresis (PAGE), SDS-PAGE, 2D-PAGE, and wet protein transfer systems are available for your needs.
Explore our rapid gel casting, electrophoresis, and transfer equipment product solutions:
Add mPAGE® Lux Casting System to your lab and have ready-to-go gels in under 3 minutes. Take a breath and get used to a fast gel casting process with less waste and without the worry of failure. That’s fresh.
The mPAGE® Lux Casting System replaces your SDS-PAGE gel casting process with faster, simpler, and safer methods with more reproducible results.
Figure 1.Auto2D® 2D-PAGE and 2D-DIGE Device.
Two-dimensional gel electrophoresis has generally been regarded as difficult to perform and time-consuming, requiring advanced user training while offering low reproducibility and high inter-operator variability.
The Auto2D® 2-D Electrophoresis Device provides a fully automatable user-friendly solution for highly reproducible, rapid results and analysis.
Discover our new mPAGE® complete solution for casting mini gels. The mPAGE® Gel Caster and Mini Gel System offer a leak-free solution for hand-casting up to 2 mini protein gels, high-resolution protein separation on precast or hand-cast gels, and electrophoretic transfer to blotting membranes. For outstanding resolution and reduced run times, use our TurboMix® Bis-Tris Acrylamide Gel Casting and mPAGE® Bis-Tris Precast Gel solutions along with the compatible mPAGE® Buffer Systems.
Western blot protocol with workflow steps for different blot procedures, describing the electrophoretic transfer of proteins from SDS polyacrylamide gels to sheets of nitrocellulose.
Protein blotting has become a routine tool used to detect low amounts of proteins in complex samples or to monitor protein expression and purification.
Western blotting (also known as immunoblotting) is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results.
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