[Determination of oleanolic acid and ursolic acid in Damnacanthus indicus from different places by RP-hPLC].

To establish A RP-HPLC method for determination of oleanolic acid and ursolic acid in Damnacanthus indicus from different places. After ultrasonic extraction with 95% ethanol,the separation was achieved on a Kromasil C18 column at 25 degrees C using methanol-2.0% phosphoric acid solution (90:10, v/v) as the mobile phase at a flow rate of 0.8 mL/min. The detection wavelength was 210 nm. Oleanolic acid showed good linear at the range of 0.124-1.24 microg (r = 0.9997). The recovery was 97.6% and RSD was 2.2%. Ursolic acid showed good linear at the range of 0.192-1.92 microg (r = 0.9999). The recovery was 102.4% and RSD was 1.9%. The proposed method is simple and accurate. It can provide reliable evidence for quality control, development and utilization of Damnacanthus indicus.