Determination of Vitamin A and E Using HPLC with UV Detection Following the Chinese National Standard (GB) Method

Introduction

Following GB method for Vitamin A and Vitamin E as it is written with a 250 x 4.6 mm Ascentis^® Express C30 column gives baseline resolution between the critical pair (gamma-, and beta-tocopherol; peaks 3 and 4). When a 150 x 4.6 mm Ascentis^® Express C30 column is used, baseline resolution is still maintained with a separation that is complete in less than half of the time of the original method. Additionally, the flow rate can be doubled with the 250 x 4.6 mm Ascentis^® Express C30 column, which gives a separation that is less than ten minutes compared to the original method. This application demonstrates how converting methods to superficially porous particle technology improves throughput.

Conclusion

Depending on the system capabilities, the separation throughput can be increased by using either a shorter length column or by doubling the flow rate.

Materials List

The column Ascentis^® Express C30 250 x 4.6 mm, 2.7 µm is available as a custom product.