- Resuspend bead pellets in 0.2 mL IP Buffer
- Verify 5% input reserved from each cell lysate and stored on ice
- Add 0.1 mL IP Buffer/cells in mild lysis or 0.6 mL/cells in harsh lysis
- Transfer diluted lysate to resuspended beads
- Incubate at 4 oC with rotation O/N
Wash
1a. For mild washing, wash 5x by adding 1mL wash buffer, vortex, spin briefly, collect beads on magnet, & remove
supernatent
1b. For harsh or stringent washing, wash 1x 1ml wash buffer, 2x 1mL stringent wash buffer, and 2x 1mL standard wash buffer