(CIP)
From Calf Intestine
(EC 3.1.3.1)
Product No. P4978
Store at –20 °C
Product Description
Product Summary
Storage buffer
Procedure: Dephosphorylation of DNA
Heat Inactivation
Materials
References
CIP is commonly used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Removal of 5’-phosphates is very useful in preventing self-ligation of cleaved DNA vectors. This property greatly reduces background (plasmids without insert) in cloning procedures.
Activity: approx. 10,000 units/mL (see label for lot specific activity)
DNase: None detected
Nickase: None detected
RNase: None detected
Unit Definition: One unit will hydrolyze 1.0 µmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C (diethanolamine).
10X CIP Buffer (C 3225)
1 M NaCl
0.5 M Tris-HCl pH 7.9 at 25 °C
0.1 M MgCl2
0.01 M dithiothreitol
10 mM Tris-HCl, pH 8.2
50 mM KCl
1 mM MgCl2
0.1 mM ZnCl2
50 % glycerol
Greater than 95% of the activity can be inactivated by heating to 75 °C for 10 minutes in the presence of 5 mM EDTA.
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