Ascentis® Express F5 (Pentafluorophenyl) U/HPLC Columns
Enhanced selectivity for stereoisomers and closely related compounds
Our Ascentis® Express F5 column brings together the highly efficient, robust Fused-Core® particle technology with F5 chemistry for the successful separation of polar and non-polar organic compounds.
The pentafluorophenylpropyl (PFP) stationary phase of the Ascentis® Express F5 column, based on Fused-Core® particle technology, provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming p-p and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis® Express F5 columns can be used for basic, acidic, or neutral compounds with alternate selectivity from C18 phases.
Learn More About Fluorinated Stationary Phases:
- Alternative Retention and Selectivity Using Fluorinated Stationary Phases
Comparision of C18 and F5 for the Separation of Closely Related Steroids |
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Featured Applications:
- Fast and Accurate LC-MS Analysis of Vitamin D Metabolites Using Ascentis® Express F5 HPLC Columns
- Anesthetics on Ascentis® Express F5
- Selegiline and Amphetamines on Ascentis® Express F5 (20/80 mobile phase mix)
- 25-dihydroxyvitamin D2, 25-hydroxyvitamin D3 and 3-epi-25-hydroxyvitamin D3 on Ascentis Express F5
- 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 on Ascentis® Express F5
- 25-hydroxyvitamin D3 and 3-epi-25-hydroxyvitamin D3 on Ascentis® Express F5
- Analysis of Steroids using Ascentis® Express F5
- Analysis of Taxols Using Ascentis® Express F5
Ascentis® Express F5 is a high-speed, high-performance liquid chromatography column based on a superficially porous Fused-Core® particle design. The Fused-Core® particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths. 3 different particle sizes are available for your specific needs:
2.0 µm particles
Best Fused-Core UHPLC Column
An optimized solution for high-throughput small molecule analysis
2.7 µm particles
Fast HPLC on ANY System
A practical solution that delivers UHPLC performance from any HPLC
5.0 µm particles
The Lab Work-Horse Column
True plug and play solution for improving existing 3 or 5 µm fully porous particle HPLC columns
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