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Medium 199 - M199 Formulations

Many early tissue culture media were predominantly formulated from animal products and/or tissue extracts.  In 1950, Morgan and his coworkers reported their efforts to produce a totally defined nutritional source for cell cultures.  Their experiments, conducted with various combinations of vitamins, amino acids, and other factors revealed that growth of explanted tissue could be measured in what has become known as Medium 199.  However, it was found that long-term cultivation of cells required addition of a serum supplement to the culture fluid.  When properly supplemented, Medium 199 (M199) has broad species applicability, particularly for cultivation of non-transformed cells.  It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

Medium 199 - M199 Formulations
L-GlutaminePhenol RedFormatSalts
  • L-Glutamine
  • Glutamine-Free
  • With Phenol Red
  • Without Phenol Red
  • Liquid
  • Powder
  • Earle’s Salts
  • Hanks’ Salts

Materials

Product NumberProduct NameProduct DescriptionPricing
C-22010Endothelial Cell Growth MediumReady-to-use kit including Basal Medium and SupplementMix, 500 ml
C-27115Chondrocyte Growth MediumBasal Medium, phenol red-free, 500 ml
211-500Endothelial Cell Growth Medium (500 ml)
M4530Medium 199With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
M5017Medium 199With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
M2520Medium 199HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
M7528Medium 199HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
M0393Medium 199With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
M2154Medium 199With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
M3769Medium 199Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture
M0650Medium 19910 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
M7653Medium 199With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
M9163Medium 19910 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
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