Dual-Labeled Probes

Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.

Choose Dual-Labeled Probes for:

• Microarray validation
• Multiply target genes / few samples
• Pathogen detection
• Multiplexing
• Viral load quantification
• Gene expression analysis
• Gene copy determination

Perfect for validating MISSION^® siRNA and shRNA knockdowns.

Benefits of Using Dual-Labeled Probes include:

• Design simplicity for sequence specificity
  
• Extensive availability of reporter/quencher combinations
  
• Increased sensitivity

Add Locked Nucleic Acid to Your Probe for:

• Increased thermal stability and hybridization specificity
• Greater accuracy in SNP detection, allele discrimination, and in vitro quantification or detection
• Easier and more sensitive probe designs for problematic target sequences 

How Dual-Labeled Probes Work

A Dual-Labeled Probe is a single-stranded oligonucleotide labeled with two different dyes. A reporter dye is located at the 5’ end and a quencher molecule located at the 3’ end. The quencher molecule inhibits the natural fluorescence emission of the reporter by fluorescence resonance energy transfer (FRET). The illustration below depicts the mechanism. 

The primer is elongated by the polymerase and the probe binds to the specific DNA template. Hydrolysis releases the reporter from the probe/target hybrid, causing an increase in fluorescence. The measured fluorescence signal is directly proportional to the amount of target DNA.

Product Features include:

• Amounts: 1, 3, 5, and 10 OD
• Purification: HPLC
• Sequence Lengths: 15 - 40 bases
• Quality Control: 100% mass spectrometry
• Format: Supplied dry in amber tubes
• Custom formats available (normalization, special plates, etc.)

Our probes are provided in a format to simplify your experimental planning.

¹JOE/TET alternative
²VIC^® alternative
³Cyanine 3 alternative
⁴TAMRA™ alternative
⁵Cyanine 5 alternative