产品名称
己糖激酶/葡萄糖-6-磷酸脱氢酶（HK / G6P-DH）, from yeast/Leuconostoc, overproducer
biological source
yeast
form
suspension
mol wt
dimer 110,000 Da
packaging
pkg of 10 mL (10737275001 [30 mg])
pkg of 5 mL (10127825001 [15 mg])
manufacturer/tradename
Roche
concentration
3.21 mg/mL
technique(s)
activity assay: suitable
color
white
pH
5.5-6.5
solubility
water: miscible
suitability
suitable for UV spectrophotometry and general use
application(s)
life science and biopharma
storage temp.
2-8°C
Quality Level
Application
己糖激酶/6-磷酸葡萄糖脱氢酶已用于测定血浆葡萄糖水平。
General description
己糖激酶负责催化糖酵解的第一步。它导致葡萄糖和 ATP 形成葡萄糖-6-磷酸和 ADP。 葡萄糖-6-磷酸脱氢酶是磷酸戊糖途径中的关键酶。负责葡萄糖-6-磷酸和 NADP +（烟酰胺腺嘌呤二核苷酸磷酸）转化为 6-磷酸葡萄糖酸内酯和 NADPH。
Other Notes
EC 2.7.1.1 & 1.1.1.49
仅用于生命科学研究。不用于诊断程序。
在+ 25°C和pH 7.6条件下，1个单位（U）HK将在1分钟内磷酸化1μmol的D-葡萄糖。
在+ 25℃和pH 7.6条件下，1单位（U）G6P-DH将在1分钟内氧化1μmol葡萄糖-6-磷酸。
在偶联试验中，每μmolD-葡萄糖磷酸化产生1μmolNADH。
体积活性：以葡萄糖和ATP为底物，+ 25℃时产生340u己糖激酶/ml。170 U葡萄糖-6-磷酸脱氢酶/ ml（+ 25°C，葡萄糖-6-磷酸为底物）
在+ 25℃和pH 7.6条件下，1单位（U）G6P-DH将在1分钟内氧化1μmol葡萄糖-6-磷酸。
在偶联试验中，每μmolD-葡萄糖磷酸化产生1μmolNADH。
体积活性：以葡萄糖和ATP为底物，+ 25℃时产生340u己糖激酶/ml。170 U葡萄糖-6-磷酸脱氢酶/ ml（+ 25°C，葡萄糖-6-磷酸为底物）
Physical form
用 3.2 M 硫酸铵溶液（pH 值约为 6）混悬。己糖激酶与 G6P-DH 混合制备。HK：G6P-DH的蛋白质含量约为2：1。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
此项目有
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