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生物来源
yeast
质量水平
形式
suspension
分子量
dimer 110,000 Da
包装
pkg of 10 mL (10737275001 [30 mg])
pkg of 5 mL (10127825001 [15 mg])
制造商/商品名称
Roche
浓度
3.21 mg/mL
技术
activity assay: suitable
颜色
white
pH值(酸碱度)
5.5-6.5
溶解性
water: miscible
适用性
suitable for UV spectrophotometry and general use
应用
life science and biopharma
储存温度
2-8°C
一般描述
己糖激酶负责催化糖酵解的第一步。它导致葡萄糖和 ATP 形成葡萄糖-6-磷酸和 ADP。 葡萄糖-6-磷酸脱氢酶是磷酸戊糖途径中的关键酶。负责葡萄糖-6-磷酸和 NADP +(烟酰胺腺嘌呤二核苷酸磷酸)转化为 6-磷酸葡萄糖酸内酯和 NADPH。
应用
己糖激酶/6-磷酸葡萄糖脱氢酶已用于测定血浆葡萄糖水平。
组分
EC 2.7.1.1 & 1.1.1.49
单位定义
在+ 25°C和pH 7.6条件下,1个单位(U)HK将在1分钟内磷酸化1μmol的D-葡萄糖。
在+ 25℃和pH 7.6条件下,1单位(U)G6P-DH将在1分钟内氧化1μmol葡萄糖-6-磷酸。
在偶联试验中,每μmolD-葡萄糖磷酸化产生1μmolNADH。
体积活性:以葡萄糖和ATP为底物,+ 25℃时产生340u己糖激酶/ml。170 U葡萄糖-6-磷酸脱氢酶/ ml(+ 25°C,葡萄糖-6-磷酸为底物)
在+ 25℃和pH 7.6条件下,1单位(U)G6P-DH将在1分钟内氧化1μmol葡萄糖-6-磷酸。
在偶联试验中,每μmolD-葡萄糖磷酸化产生1μmolNADH。
体积活性:以葡萄糖和ATP为底物,+ 25℃时产生340u己糖激酶/ml。170 U葡萄糖-6-磷酸脱氢酶/ ml(+ 25°C,葡萄糖-6-磷酸为底物)
外形
用 3.2 M 硫酸铵溶液(pH 值约为 6)混悬。己糖激酶与 G6P-DH 混合制备。HK:G6P-DH的蛋白质含量约为2:1。
其他说明
仅用于生命科学研究。不用于诊断程序。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
常规特殊物品
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