C0646
Cytodex ® 1 微载体微球
Cytodex 1, 60-87 μm
material
dextran beads
sterility
non-sterile
particle size
60-87 μm
density
1.03 g/cm3 at 25 °C
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General description
一种通用的微载体，适用于大多数已建立的锚着依存性细胞系。
锚着依存性细胞的高产培养：微载体培养是一种有望实现锚着依存性细胞高产培养的实用技术。Cytodex专为培养从几毫升到6000升以上的各种动物细胞而开发的。在简单的悬浮培养系统中使用Cytodex可提供每mL数百万个细胞的产量。
适用于大多数已建立的细胞系：由分布在整个基质中的带正电荷DEAE*基团取代交联右旋糖酐基质而形成，是一种通用微载体。特别适用于大多数已建立的细胞系，以及从原代细胞和正常二倍体细胞株培养物中生产病毒或细胞产物。
锚着依存性细胞的高产培养：微载体培养是一种有望实现锚着依存性细胞高产培养的实用技术。Cytodex专为培养从几毫升到6000升以上的各种动物细胞而开发的。在简单的悬浮培养系统中使用Cytodex可提供每mL数百万个细胞的产量。
适用于大多数已建立的细胞系：由分布在整个基质中的带正电荷DEAE*基团取代交联右旋糖酐基质而形成，是一种通用微载体。特别适用于大多数已建立的细胞系，以及从原代细胞和正常二倍体细胞株培养物中生产病毒或细胞产物。
Application
用于一般微载体培养。
本品溶胀于液体溶液时（如使用），平均粒径为 190 μm。60-87 μm 的粒度与干燥形式的微珠有关。
本品溶胀于液体溶液时（如使用），平均粒径为 190 μm。60-87 μm 的粒度与干燥形式的微珠有关。
Features and Benefits
- 通用微载体，适用于大多数已建立的锚着依存性细胞系。
- 在整个微孔基质中包含正电荷。
- 透明，便于显微镜检查附着细胞。
Preparation Note
使用前，将微球溶于 PBS (50 mL/g) 中，并在 120°C 下高压灭菌。
Other Notes
含正电荷 DEAE 基团的葡聚糖珠。
Legal Information
Cytodex is a registered trademark of Cytiva
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Jan Cedergren et al.
The Journal of rheumatology, 34(11), 2162-2170 (2007-10-17)
To compare total and intracellular oxidative activation of blood and synovial fluid (SF) neutrophils from patients with rheumatoid arthritis (RA) and other arthritides with blood donor neutrophils. Peripheral blood and SF samples were obtained from 26 gonarthritis patients (13 RA
Elsa Abranches et al.
Biotechnology and bioengineering, 96(6), 1211-1221 (2006-09-29)
Embryonic stem (ES) cells have been shown to differentiate in vitro into a wide variety of cell types having significant potential for tissue regeneration. Therefore, the operational conditions for the ex vivo expansion and differentiation should be optimized for large-scale
Sónia Sá Santos et al.
Journal of neuroscience research, 79(1-2), 26-32 (2004-12-17)
We report the first approach for growth and maintenance of primary astrocytes on a fully controlled environment. For this purpose, cells were immobilized in Cytodex microcarriers and grown in a stirred tank bioreactor. The distribution of astrocytes at the microcarrier
Kamla Dutt et al.
Current stem cell research & therapy, 6(4), 350-361 (2011-04-07)
Retinal degenerations are the leading cause of genetically inherited blindness. One of the strategies currently being tested for the treatment is cell/tissue transplantation. As such stem cells and tissue engineered constructs are of great importance. This report describes the growth
A M Fernandes et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 42(6), 515-522 (2009-05-19)
Future clinical applications of human embryonic stem (hES) cells will require high-yield culture protocols. Currently, hES cells are mainly cultured in static tissue plates, which offer a limited surface and require repeated sub-culturing. Here we describe a stirred system with
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