C0646
Cytodex ® 1 微载体微球
Cytodex 1, 60-87 μm
material
dextran beads
sterility
non-sterile
particle size
60-87 μm
density
1.03 g/cm3 at 25 °C
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General description
一种通用的微载体，适用于大多数已建立的锚着依存性细胞系。
锚着依存性细胞的高产培养：微载体培养是一种有望实现锚着依存性细胞高产培养的实用技术。Cytodex专为培养从几毫升到6000升以上的各种动物细胞而开发的。在简单的悬浮培养系统中使用Cytodex可提供每mL数百万个细胞的产量。
适用于大多数已建立的细胞系：由分布在整个基质中的带正电荷DEAE*基团取代交联右旋糖酐基质而形成，是一种通用微载体。特别适用于大多数已建立的细胞系，以及从原代细胞和正常二倍体细胞株培养物中生产病毒或细胞产物。
锚着依存性细胞的高产培养：微载体培养是一种有望实现锚着依存性细胞高产培养的实用技术。Cytodex专为培养从几毫升到6000升以上的各种动物细胞而开发的。在简单的悬浮培养系统中使用Cytodex可提供每mL数百万个细胞的产量。
适用于大多数已建立的细胞系：由分布在整个基质中的带正电荷DEAE*基团取代交联右旋糖酐基质而形成，是一种通用微载体。特别适用于大多数已建立的细胞系，以及从原代细胞和正常二倍体细胞株培养物中生产病毒或细胞产物。
Application
用于一般微载体培养。
本品溶胀于液体溶液时（如使用），平均粒径为 190 μm。60-87 μm 的粒度与干燥形式的微珠有关。
本品溶胀于液体溶液时（如使用），平均粒径为 190 μm。60-87 μm 的粒度与干燥形式的微珠有关。
Features and Benefits
- 通用微载体，适用于大多数已建立的锚着依存性细胞系。
- 在整个微孔基质中包含正电荷。
- 透明，便于显微镜检查附着细胞。
Preparation Note
使用前，将微球溶于 PBS (50 mL/g) 中，并在 120°C 下高压灭菌。
Other Notes
含正电荷 DEAE 基团的葡聚糖珠。
Legal Information
Cytodex is a registered trademark of Cytiva
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Christopher J Hewitt et al.
Biotechnology letters, 33(11), 2325-2335 (2011-07-20)
The effects on human mesenchymal stem cell growth of choosing either of two spinner flask impeller geometries, two microcarrier concentrations and two cell concentrations (seeding densities) were investigated. Cytodex 3 microcarriers were not damaged when held at the minimum speed
Denver C Surrao et al.
Tissue engineering. Part A, 17(15-16), 1959-1967 (2011-04-01)
Tissue engineering is a promising approach for articular cartilage repair; however, it is challenging to produce adequate amounts of tissue in vitro from the limited number of cells that can be extracted from an individual. Relatively few cell expansion methods
Pierre Danhier et al.
PloS one, 8(1), e53324-e53324 (2013-02-06)
Cell detachment is a procedure routinely performed in cell culture and a necessary step in many biochemical assays including the determination of oxygen consumption rates (OCR) in vitro. In vivo, cell detachment has been shown to exert profound metabolic influences
C Hagen et al.
Journal of microscopy, 230(Pt 2), 288-296 (2008-05-01)
A method is described employing microcarrier spheres of cross-linked dextran for obtaining ultra- and semithin vitreous sections from high-pressure frozen anchorage-dependent (mammalian) cells. Avoiding trypsination or scraping cells off from the culture surface, the presented approach allows for cryoimmobilization, cryosectioning
S Mehdi Hassanzadeh et al.
Pakistan journal of biological sciences : PJBS, 14(7), 441-448 (2011-09-10)
To achieve higher titer of rabies virus higher density of host cells will need. In this study, capability of FibraCel disks packed in 500 mL spinner basket versus Cytodex-1 in 500 mL spinner flask was investigated for propagation of Vero
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