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Merck
CN

MITOISO2

线粒体分离试剂盒

sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

别名:

线粒体提取试剂盒, 线粒体纯化试剂盒

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关于此项目

NACRES:
NA.32
UNSPSC Code:
12352200
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产品名称

线粒体分离试剂盒, sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

usage

sufficient for 50 applications (2-5 x 107 cells)

technique(s)

ELISA: suitable
western blot: suitable

shipped in

dry ice

storage temp.

−20°C

Quality Level

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Application

线粒体分离试剂盒用于分离下列来源中的线粒体:
  • Ewing′肉瘤肿瘤家族(ESFT)细胞
  • 肺结核患者血液样本
  • 人神经母细胞瘤细胞和神经胶质瘤细胞
  • 肌小管
  • HepG2细胞

Features and Benefits

  • 该试剂盒允许快速和简单地从细胞中提取浓缩的线粒体部分。
  • 分离的线粒体大多具有完整的内外膜。
  • 可用于提取线粒体蛋白进行蛋白质组分析

General description

线粒体是一种双层膜结构,是真核细胞中大部分能量产生的位点。线粒体分离试剂盒包含两种缓冲液:一种用于提取功能研究的蛋白质,另一种用于蛋白质组学研究的分析。此外,该试剂盒还包括保存完整线粒体的存储缓冲液。

Other Notes

  • 本产品仅供研发使用,不用于药物、家庭或其他用途。
  • 请查阅材料安全数据表,了解有关危险和安全处理方法的信息。
  • 使用超纯水制备试剂。
  • 请参阅产品信息表以了解有关试剂盒成分、所需试剂、存储和稳定性以及隔离方案的更多信息。

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2

存储类别

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

法规信息

低风险生物材料
常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Vladislav V Glinsky et al.
Neoplasia (New York, N.Y.), 11(9), 901-909 (2009-09-03)
At present, there is no efficient curative therapy for cancer patients with advanced metastatic disease. Targeting of antiapoptotic molecules acting on the mitochondrial apoptosis pathway could potentially augment antimetastatic effect of cytotoxic drugs. Similarly to Bcl-2 family members, beta-galactoside-binding lectin
Youguang Gao et al.
Journal of translational medicine, 18(1), 114-114 (2020-03-07)
We have reported that polydatin (PD) alleviates mitochondrial dysfunction in rat models of sepsis-induced acute kidney injury (SI-AKI), but the mechanism is not well understood. Here, we investigated the role of Parkin-mediated mitophagy in the protective effects of PD in
Mitochondria
Cooper G M
Journal of biological and chemical chronicles (2000)
Theodora Panagaki et al.
Biomolecules, 10(4) (2020-04-29)
Down syndrome (trisomy of human chromosome 21) is a common genetic disorder. Overproduction of the gaseous mediator hydrogen sulfide (H2S) has been implicated in the pathogenesis of neurological and metabolic deficits associated with Down syndrome. Several lines of data indicate
Detection and characterisation of multi-drug resistance protein 1 (MRP-1) in human mitochondria
Roundhill, EA and Burchill, SA
British Journal of Cancer, 106(6), 1224-1224 (2012)

商品

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。

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