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Merck
CN

W1754

Sigma-Aldrich

PCR Reagent, suitable for PCR

别名:

H2O

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关于此项目

线性分子式:
H2O
化学文摘社编号:
分子量:
18.02
Beilstein:
2050024
EC 号:
MDL编号:
UNSPSC代码:
12191602
PubChem化学物质编号:
NACRES:
NA.25
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产品名称

水, PCR Reagent

蒸汽密度

<1 (vs air)

质量水平

蒸汽压

3 mmHg

无菌性

sterile-filtered

表单

liquid

包装

vial of 1.5 mL

技术

PCR: suitable

折射率

n20/D 1.34 (lit.)

pH值(酸碱度)

5-7

沸点

100 °C (lit.)

mp

0 °C (lit.)

密度

1.000 g/mL at 3.98 °C (lit.)

异质活性

DNase, none detected
RNase, none detected

SMILES字符串

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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一般描述

对 PCR 级水进行无菌过滤。它不含核酸外切酶(dna 酶、rna 酶)和核酸内切酶(切口酶),也不含核酸污染。

应用

水已用于:

  • 作为反应混合物的组分和微流控RT-qPCR的稀释剂
  • 作为反应混合物的组分,以扩增真菌(杂色云芝)DNA的产物
  • 作为稀释剂和反应混合物的组分,以扩增cDNA
  • 水已用于在聚合酶链式反应 (PCR) 中补充样品的最终体积
适用于聚合酶链式反应 (PCR)

其他说明

便于将水产品规格与 水规格表进行比较

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

N Wellinghausen et al.
Applied and environmental microbiology, 67(9), 3985-3993 (2001-08-30)
Contamination of hospital water systems with legionellae is a well-known cause of nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for quantitative determination of legionellae in potable water samples. Primers that amplify both a 386-bp fragment of the
Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
Bolton DJ, et al.
Journal of Applied Microbiology, 111(2), 484-490 (2011)
Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
Matthew J Ellington et al.
The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)
If you?ve seen one worm, have you seen them all? Spatial, community, and genetic variability of tubificid communities in Montana
Lodh N, et al.
Freshwater science, 34(3), 909-917 (2015)
Francine Marciano-Cabral et al.
Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)
The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and

商品

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

实验方案

Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

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