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Merck
CN

W1754

水

PCR Reagent, suitable for PCR

别名:

H2O

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关于此项目

线性分子式:
H2O
化学文摘社编号:
7732-18-5
分子量:
18.02
NACRES:
NA.25
PubChem Substance ID:
24900779
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
MFCD00011332
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg

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产品名称

水, PCR Reagent

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

InChI

1S/H2O/h1H2

SMILES string

O

vapor density

<1 (vs air)

vapor pressure

3 mmHg

sterility

sterile-filtered

form

liquid

packaging

vial of 1.5 mL

technique(s)

PCR: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, none detected
RNase, none detected

Quality Level

200

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相关类别

Application

水已用于：

  • 作为反应混合物的组分和微流控RT-qPCR的稀释剂
  • 作为反应混合物的组分，以扩增真菌（杂色云芝）DNA的产物
  • 作为稀释剂和反应混合物的组分，以扩增cDNA
  • 水已用于在聚合酶链式反应 (PCR) 中补充样品的最终体积
适用于聚合酶链式反应 (PCR)

General description

对 PCR 级水进行无菌过滤。它不含核酸外切酶（dna 酶、rna 酶）和核酸内切酶（切口酶），也不含核酸污染。

Other Notes

便于将水产品规格与 水规格表进行比较。

存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000423209
0000423207
0000423208
0000423207
0000423208

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Circulating Tfh1 (cTfh1) cell numbers and PD1 expression are elevated in low-grade B-cell non-Hodgkin?s lymphoma and cTfh gene expression is perturbed in marginal zone lymphoma
Elliot T B, et al.
PLoS ONE (2018)
J Loeffler et al.
Journal of clinical microbiology, 37(4), 1200-1202 (1999-03-13)
Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed.
Francine Marciano-Cabral et al.
Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)
The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and
Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
Bolton DJ, et al.
Journal of Applied Microbiology, 111(2), 484-490 (2011)
N Wellinghausen et al.
Applied and environmental microbiology, 67(9), 3985-3993 (2001-08-30)
Contamination of hospital water systems with legionellae is a well-known cause of nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for quantitative determination of legionellae in potable water samples. Primers that amplify both a 386-bp fragment of the
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实验方案

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

Antibody-Enzyme Mediated Hot Start PCR Protocol

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

Hot Start dNTP Protocol

Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.

Amplification of Genomic DNA using REDTaq® DNA Polymerase

Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.

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