159220
焦碳酸二乙酯
96% (NT)
别名:
DEP, DEPC, 氧二甲酸二乙酯, 焦炭酸二乙酯, 重碳酸二乙酯
质量水平
方案
96% (NT)
表单
liquid
折射率
n20/D 1.398 (lit.)
沸点
93-94 °C/18 mmHg (lit.)
密度
1.101 g/mL at 25 °C (lit.)
官能团
carbonate
运输
wet ice
储存温度
2-8°C
SMILES字符串
CCOC(=O)OC(=O)OCC
InChI
1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
InChI key
FFYPMLJYZAEMQB-UHFFFAOYSA-N
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一般描述
焦碳酸二乙酯选择性地与十字形结构的单链环反应。已有研究表明焦碳酸二乙酯可使核糖核酸酶发生不可逆失活。
应用
焦碳酸二乙酯被用作：
- 核酸酶抑制剂，用于从黄化和绿色植物组织中提取未降解的核酸
- 抗微生物添加剂
- 核糖核酸酶抑制剂
- 组氨酸残基修饰剂
- 将亚胺转化为氨基甲酸酯的试剂
蛋白中His及Tyr残基的修饰试剂。用于dsDNA结构破坏的稳健探针，可与完全或部分未堆叠的碱基发生反应。
生化/生理作用
在溶液中浓度约为 0.1% (v/v) 时可使 RNase 失活，从而防止 RNA 被降解。
警示用语：
Warning
危险声明
危险分类
Acute Tox. 4 Oral
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
156.2 °F - closed cup
闪点(°C)
69 °C - closed cup
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Tetrahedron, 48, 3445-3445 (1992)
J C Furlong et al.
Nucleic acids research, 14(10), 3995-4007 (1986-05-27)
Diethyl pyrocarbonate reacts with the single-stranded loops of cruciform structures with great selectivity. Adenine bases are carbethoxylated, as a result of which the backbone may be cleaved with piperidine, and the level of chemical modification at each base may be
Food Sci. Technol., 57, 343-343 (1993)
P D Pelton et al.
The Journal of biological chemistry, 267(9), 5916-5920 (1992-03-25)
The pH dependence of myo-inositol monophosphatase may indicate a role for histidine residues in the catalytic mechanism (Ganzhorn, A. J., and Chanal, M.-C. (1990) Biochemistry 29, 6065-6071). This possibility was investigated by chemical modification. At pH 6.0 and 25 degrees
A mechanism of the irreversible inactivation of bovine pancreatic ribonuclease by diethylpyrocarbonate. A general reaction of diethylpyrocarbonate . A general reaction of diethylpyrocarbonate with proteins.
B Wolf et al.
European journal of biochemistry, 13(3), 519-525 (1970-04-01)
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