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Merck
CN

200182

Sigma-Aldrich

Nitrazine Yellow

indicator grade, Dye content 85 %

别名:

2-(2,4-Dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt, 2-(2,4-Dinitrophenylazo)naphthol-3,6-disulfonic acid disodium salt, Nitrazol Yellow

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关于此项目

经验公式（希尔记法）:
C16H8N4Na2O11S2
化学文摘社编号:
5423-07-4
分子量:
542.36
颜色索引号:
14890
EC 号:
226-549-8
MDL编号:
MFCD00003938
UNSPSC代码:
12171500
PubChem化学物质编号:
24851961
NACRES:
NA.47

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等级

indicator grade

方案

>90.0% (HPLC)

表单

powder

组成

Dye content, 85%

溶解性

H2O: 1 mg/mL

λmax

586 nm

ε (消光系数)

≥35000 at 587-591 nm in 0.1 M NaOH at 0.01 g/L

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2[N+]([O-])=O)[N+]([O-])=O)c(cc3cc(ccc13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C16H10N4O11S2.2Na/c21-16-11-3-2-10(32(26,27)28)5-8(11)6-14(33(29,30)31)15(16)18-17-12-4-1-9(19(22)23)7-13(12)20(24)25;;/h1-7,21H,(H,26,27,28)(H,29,30,31);;/q;2*+1/p-2/b18-17+;;

InChI key

XBLIFEQTVVSTIM-QIKYXUGXSA-L

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应用

Nitrazine Yellow has been used in dye-protein binding assay.

生化/生理作用

Nitrazine Yellow ( 2-(2,4-dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt) is a pH-sensitive azo dye. It changes color from yellow to blue in pH range 6.0–7.0 and is widely used in microscopy. Throat swab immersed in nitrazine yellow is used to identify pH variations in the vagina before and after amniotomy.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCD1402
MKBQ6886V
MKBG8083V
MKBD9527V
36296KJ

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Gilbert G G Donders et al.
Infectious diseases in obstetrics and gynecology, 2010, 504972-504972 (2010-10-19)
Accessing vaginal pH is fundamental during gynaecological visit for the detection of abnormal vaginal flora (AVF), but use of pH strips may be time-consuming and difficult to interpret. The aim of this study was to evaluate the VS-SENSE test (Common
Structural characterisation of Nitrazine Yellow by NMR spectroscopy
Viscardi G
Dyes and Pigments, 27, 87- 95 (2003)
USE OF THE NITRAZINE YELLOW SWAB TEST IN THE DIAGNOSIS OF RUPTURED MEMBRANES
Mills AM
British Journal of Obstetrics and Gynaecology, 84, 134-180 (1977)
"Fig leaf" ferning and positive Nitrazine testing: semen as a cause of misdiagnosis of premature rupture of membranes.
J A McGregor et al.
American journal of obstetrics and gynecology, 151(8), 1142-1143 (1985-04-15)
The influence of a polyamide matrix on the halochromic behaviour of the pH-sensitive azo dye Nitrazine Yellow
Schueren L V D
Dyes and Pigments, 94, 443-451 (2012)
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