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Merck
CN

216720

Sigma-Aldrich

4′,5′-二溴荧光素

Dye content 95 %

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别名:
溶剂红 72
经验公式(希尔记法):
C20H10Br2O5
CAS号:
分子量:
490.10
颜色索引号:
45370:1
Beilstein:
57189
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47

检测方案

>95% (TLC)

质量水平

形式

powder

组成

Dye content, 95%

mp

270-273 °C (lit.)

λmax

450 nm

ε (消光系数)

≥5000 at 445-451 nm
≥50000 at 227-233 nm

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

Oc1ccc2c(Oc3c(Br)c(O)ccc3C24OC(=O)c5ccccc45)c1Br

InChI

1S/C20H10Br2O5/c21-15-13(23)7-5-11-17(15)26-18-12(6-8-14(24)16(18)22)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

InChI key

ZDTNHRWWURISAA-UHFFFAOYSA-N

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一般描述

4′,5′-Dibromofluorescein (DBF) is a fluorone dye. Fluorone dyes are hydroxyl-xanthene dyes (homologues of fluorescein) and have been the most widely used class of organic fluorophores for labeling and sensing biomolecules. DBF is routinely used as a ligand for studying the properties of protein using spectral analysis.

应用

4′,5′-Dibromofluorescein has been used to stain proteins in 1-D and 2-D sodium dodecyl sulfatepolyacrylamide gel electrophoresis SDS-PAGE. This method is rapid, economical, and highly sensitive in comparison with other proteomic research staining methods.

象形图

Skull and crossbones

警示用语:

Danger

危险声明

预防措施声明

危险分类

Acute Tox. 3 Oral

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges


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