40718
焦碳酸二乙酯
96% (NT)
别名:
DEP, DEPC, 氧二甲酸二乙酯, 焦炭酸二乙酯, 重碳酸二乙酯
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关于此项目
线性分子式:
O(COOC2H5)2
化学文摘社编号:
分子量:
162.14
UNSPSC Code:
12352100
NACRES:
NA.22
PubChem Substance ID:
EC Number:
216-542-8
Beilstein/REAXYS Number:
637031
MDL number:
Assay:
96% (NT)
Form:
liquid
产品名称
焦碳酸二乙酯, 96% (NT)
InChI
1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
SMILES string
CCOC(=O)OC(=O)OCC
InChI key
FFYPMLJYZAEMQB-UHFFFAOYSA-N
assay
96% (NT)
form
liquid
color
APHA: ≤20
refractive index
n20/D 1.398 (lit.)
n20/D 1.398
bp
93-94 °C/18 mmHg (lit.)
solubility
95% ethanol: 4.5 g/10 mL, clear, colorless
density
1.121 g/mL at 20 °C
1.101 g/mL at 25 °C (lit.)
functional group
carbonate
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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Application
焦碳酸二乙酯可用于以下研究：
- 用于修饰蛋白质中的组氨酰残基。
- 作为核酸酶抑制剂，用于从黄化和绿色植物组织中提取未降解的核酸。
- 用于修饰线性和超螺旋质粒 DNA。
- 作为研究负超螺旋 DNA 二级结构的化学探针。
- 用于肌动蛋白的组氨酸残基的乙氧基化。
蛋白中His及Tyr残基的修饰试剂。用于dsDNA结构破坏的稳健探针，可与完全或部分未堆叠的碱基发生反应。
Biochem/physiol Actions
在溶液中浓度约为 0.1% (v/v) 时可使 RNase 失活，从而防止 RNA 被降解。
General description
焦碳酸二乙酯（DEP）是一种杀菌剂，在水中分解成 CO2 和乙醇。DEP 抑制酶（如 DNA 酶和 RNA 酶），而不影响核酸。它可用于从组织制备 mRNA。
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
156.2 °F - closed cup
flash_point_c
69 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Effects of diethyl pyrocarbonate and methyl methanesulfonate on nucleic acids and nucleases.
I Fedorcsák et al.
Acta chemica Scandinavica, 20(1), 107-112 (1966-01-01)
Modification of histidyl residues in proteins by diethylpyrocarbonate.
E W Miles
Methods in enzymology, 47, 431-442 (1977-01-01)
Selective carbethoxylation of the histidine residues of actin by diethylpyrocarbonate.
G Hegyi et al.
European journal of biochemistry, 44(1), 7-12 (1974-05-02)
W Herr
Proceedings of the National Academy of Sciences of the United States of America, 82(23), 8009-8013 (1985-12-01)
Purine residues located within regions of DNA that have the potential to form left-handed Z-helical structures are modified preferentially by diethyl pyrocarbonate; this hyperreactivity is dependent on the degree of negative superhelicity of the circular DNA molecules. As negative superhelical
A new method based on the use of diethyl pyrocarbonate as a nuclease inhibitor for the extraction of undegraded nucleic acid from plant tissues.
F Solymosy et al.
European journal of biochemistry, 5(4), 520-527 (1968-09-24)
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