4-Aminoindole is an indole derivative. Its cytokinin activity has been assessed by tobacco pith callus bioassay. 4-Aminoindole can be prepared by reacting 2,6-dinitrotoluene and N,N-dimethylformamide dimethylacetal in anhydrous DMF.

4-Aminoindole may be used to synthesize:

• macrolactam tumour promoter indolactam V
• tricyclic structure of 2-substituted-pyrrolo[2,3-h]quinolin-4-one
• 4-azidoindole

Reactant for preparation of:

• Inhibitors of bacterial thymidylate synthase
• Mimetics of non-alkaloid toxin lignan anticancer and antiviral agent Podophyllotoxin (PPT)
• Inhibitors of Gli1-mediated transcription in the Hedgehog pathway
• Protein kinase C θ (PKCθ) inhibitors
• Indolic non-peptidic HIV protease inhibitors
• Transient receptor potential cation channel subfamily V member 1 (TRPV1) antagonists
• Cyclooxygenase-2 (COX-2) and lipoxygenase (LOX) inhibitors
• 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) inhibitors
• Short chain 4-substituted indoles as potent αvβ3 antagonist
• Ligands of serotonin transporter and 5-HT1A receptors