NanoFabTx^™ DBCO Lipid Mix

NanoFabTx^™ DBCO Lipid Mix is a ready-to-use nanoformulation lipid mixture for the synthesis of DBCO-functionalized liposomes. This lipid mix enables users to encapsulate a wide variety of small molecules for targeting and immunotherapy applications without the need for lengthy trial-and-error optimization.

This product includes a curated lyophilized lipid mixture containing rationally selected lipids in precise ratios that have been optimized to achieve a desired size range of liposomes. Step-by-step protocols for the synthesis of liposomes surface-functionalized with DBCO groups using extrusion and microfluidics are also included. The synthesized DBCO-functionalized liposomes enable the conjugation of azide-tagged antibodies or proteins to the liposomes surface for immunothereapy applications and multi-targeting or multi-type cargo delivery.

• A ready-to-use nanoformulation lipid blend for the synthesis of DBCO-functionalized liposomes
• Step-by-step extrusion and microfluidics protocols for liposome synthesis developed and tested by our formulation scientists
• Flexible synthesis methods to create uniform and reproducible liposomes
• Protocols and lipid blend optimized to make liposomes around 100 nm diameter with low polydispersity
• Lipid mix optimized for azide-tagged protein or antibody conjugation and small molecule encapsulation

Comprehensive protocols for liposome synthesis are included:

• A lipid film hydration and extrusion protocol
• A microfluidics protocol using commercial platforms or syringe pumps

The microfluidics protocol included with this product uses the NanoFabTx^™ device kit (911593). These kits come with the microfluidics chips, fittings, and tubing required to get started with microfluidics-based synthesis (compatible microfluidics system or syringe pump required).

For more information, please refer to the protocol under the Protocol section of this page.

NANOFABTX is a trademark of Sigma-Aldrich Co. LLC