This reagent enables the specific labeling of various alkynylated molecules, such as DNA, oligonucleotides, and proteins, with biotin. The binding of biotin to avidin or streptavidin can be employed in downstream affinity applications, such as the isolation of biotinylated molecules or their interaction with streptavidin conjugates. Biotin azide undergoes a copper-catalyzed click reaction with terminal alkynes, enabling the incorporation of biotin and biotin derivatives into biomolecules that contain alkyne groups through azide-alkyne cycloaddition.

Biotin-azide (N-(3-Azidopropyl)biotinamide) is an azido derived biotin probe. Biotin-azide can be used to prepare various biotinylated conjugates via Click Chemistry.The conjugation of biotin and its derivatives to various biomolecules can be achieved through the widely recognized click chemistry methodology, followed by their detection using streptavidin, avidin, or NeutrAvidin biotin-binding proteins. Biotin azide serves as a valuable reagent for the synthesis of diverse biotinylated conjugates via Click Chemistry