General description
Protein delivery system, Human BMP-2, PODS contains the polyhedrin protein co-crystalized with Human BMP-2 (Bone Morphogenetic Protein-2). BMP-2 functions as a potent inducer of bone and cartilage development. Active BMP-2 consists of forming a homodimer or a heterodimer with a related BMP, such as BMP-7. BMP-2 signals through type I and type II receptor tyrosine kinases in conjunction with SMAD proteins to directly promote osteoblast differentiation. BMP-2 is also important during cardiac development and supports epicardial cell migration.
Application
PODS are composed of a bioactive cargo protein within a polyhedrin crystal lattice, with polyhedrin as the main protein. The 25 µg SKU size indicates the amount of bioactive cargo, typically 0.5% to 5% of the total protein. For instance, a 25 µg SKU at a 2% cargo loading contains 1.25 mg of total protein. PODS enable sustained release of cargo proteins when proteases degrade the polyhedrin scaffold. Growth factors are not bioavailable until released, and their concentration in media depends on the amount added and the release rate. In 10% serum, peak levels are usually reached within 24-48 hours, with about 20% of the cargo becoming soluble.
PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.
PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.
Features and Benefits
- Protein delivery system, PODS Human BMP-2, PODS comes with molecular weight of 99.8 kDa, length 397 aa, with endotoxin level of <0.06 EU/ml as measured by gel clot LAL assay. PODS co-crystals may be reconstituted at 200 million co-crystals/ml in sterile PBS. 20% glucose has a buoyant density closer to PODS co-crystals and can be useful for aliquoting.
- This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols. PODS co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).
- PODS provides durability, stability AND sustained release, addressing the limitations of conventional and enabling researchers to effortlessly control and manipulate cells in 2D and 3D culture as well as in-vivo.
Legal Information
PODS is a registered trademark of Cell Guidance Systems Ltd
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Enhanced in vivo retention of low dose BMP-2 via heparin microparticle delivery does not accelerate bone healing in a critically sized femoral defect
Hettiaratchi H M, et al.
Acta Biomaterialia, 59, 21-32 (2017)
Enhancing BMP-2-mediated osteogenesis with a synthetic heparan sulfate mimetic
Biomaterials advances, 155, 213671-213671 (2023)
Formulation, Delivery and Stability of Bone Morphogenetic Proteins for Effective Bone Regeneration
Bialy I E, et al.
Pharmaceutical Research, 34, 1152?1170-1152?1170 (2017)
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