biological source
(Spodoptera frugiperda (Sf9) cell culture)
Quality Level
form
(monodisperse crystals)
packaging
1 ea of 25 μg
technique(s)
: suitable using 3D bioprinting | cell culture
impurities
<0.06 EU/mL Endotoxin
color
colorless
storage temp.
2-8°C
General description
Protein delivery system, TGF-β3, PODS contains the polyhedrin protein co-crystalized with Human TGF-β3. Transforming Growth Factors (TGFs) are multifunctional peptides that regulate growth and differentiation in most cell types. The TGF-β family of proteins signal through serine/threonine kinase receptors. TGF-β isoforms (TGF-β1, -β2, and -β3) have overlapping, yet distinct biological actions in developing and adult tissues. TGF-β3 is an important factor in regulating cell adhesion and accelerating wound repair. TGF-β3 also functions during osteoblast proliferation, chemotaxis, and collagen synthesis.
Application
PODS are composed of a bioactive cargo protein within a polyhedrin crystal lattice, with polyhedrin as the main protein. The 25 µg SKU size indicates the amount of bioactive cargo, typically 0.5% to 5% of the total protein. For instance, a 25 µg SKU at a 2% cargo loading contains 1.25 mg of total protein.PODS enable sustained release of cargo proteins when proteases degrade the polyhedrin scaffold. Growth factors are not bioavailable until released, and their concentration in media depends on the amount added and the release rate. In 10% serum, peak levels are usually reached within 24-48 hours, with about 20% of the cargo becoming soluble.
PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically
PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically
Features and Benefits
- Protein delivery system, PODS human TGF-β3 comes with molecular weight of 36 kDa, length 158 aa and endotoxin level <0.06 EU/ml. PODS co-crystals may be reconstituted at 200 million co-crystals/ml in sterile PBS. 20% glucose has a buoyant density closer to PODS co-crystals and can be useful for aliquoting.
- This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols. PODS co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).
- PODS provides durability, stability and sustained release, addressing the limitations of conventional and enabling researchers to effortlessly control and manipulate cells in 2D and 3D culture as well as in-vivo.
Legal Information
PODS is a registered trademark of Cell Guidance Systems Ltd
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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Explore PODS® crystals for sustained, localized protein delivery, enhancing in vitro models, stem cell culture, and regenerative medical research.
Effect of locally applied transforming growth factor Beta3 on wound healing and stenosis development in tracheal surgery
Elicora A, et al.
Respiratory Care, 59(8), 1281-1286 (2014)
TGF-beta 3 expression during umbilical cord development and its alteration in pre-eclampsia
Copland B I, et al.
Placenta, 23(4), 311-321 (2002)
The Role of TGF-?3 in Radiation Response
Hanson I, et al.
International Journal of Molecular Sciences, 24(8), 7614-7614 (2023)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 939250-1EA | 04065271982000 |