ETT 激活剂

Name: ETT 激活剂
Brand: ALDRICH

0.25M

别名:

5-乙硫基四氮唑溶液, 5-（乙硫基）-1 H-四唑, 5-（乙硫基）四唑溶液

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关于此项目

UNSPSC Code:

41116105

NACRES:

NA.51

MDL number:

MFCD00068733

Form:

liquid

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属性

Quality Level

200

type

for DNA synthesis

product line

Proligo Reagents

form

liquid

concentration

32-35 g/L (titration)

impurities

≤30 ppm water content (Karl Fischer)

SMILES string

CCSc1nnn[nH]1

InChI

1S/C3H6N4S/c1-2-8-3-4-6-7-5-3/h2H2,1H3,(H,4,5,6,7)

InChI key

GONFBOIJNUKKST-UHFFFAOYSA-N

说明

Preparation Note

Acetonitrile / 5-(Ethylthio)-1H-Tetrazole 100 / 3 (v/w)

Other Notes

Activator solution, containing 5-(Ethylthio)-1H- tetrazole (ETT) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.