type
for DNA synthesis
product line
Proligo Reagents
form
liquid
storage condition
dry at room temperature
concentration
88.7-108.4 g/L (Acetic Anhydride content, titration)
color
clear
compatibility
configured for PerkinElmer, configured for Polygen
Quality Level
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General description
Cap A solution contains acetic anhydride and tetrahydrofuran (THF). It is used after the phosphoramidite reaction/coupling step.
Biochem/physiol Actions
Cap A solution aborts chains bearing unreacted 5′ hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′ hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.
Preparation Note
乙酸酐/四氢呋喃 9.1/90.9 (v/v)
signalword
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system, Respiratory system
supp_hazards
存储类别
3 - Flammable liquids
wgk
WGK 1
flash_point_f
-6.2 °F - closed cup
flash_point_c
-21.2 °C - closed cup
法规信息
易制毒化学品（2类）
危险化学品
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