type
for DNA synthesis
Quality Segment
product line
Proligo Reagents
form
liquid
storage condition
dry at room temperature
technique(s)
DNA synthesis: suitable
impurities
≤200 ppm water content (Karl Fischer)
color
clear
suitability
conforms to structure (GC, Formulation)
storage temp.
room temp
General description
Cap 1 solution consists of n-methylimidazole, lutidine, and acetonitrile (ACN).
Application
Cap 1 is suitable for use after the phosphoramidite reaction/coupling step.
Biochem/physiol Actions
Cap 1 solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.
Preparation Note
Acetonitrile / Lutidine / N-Methylimidazole 8/1/1 (v/v/v)
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警示词
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Repr. 2 - Skin Corr. 1B
存储类别
3 - Flammable liquids
闪点 (F)
35.6 °F - closed cup
闪点 (°C)
2.0 °C - closed cup