100600C
Avanti
E. coli Extract Polar
Avanti Research™ - A Croda Brand
别名:
E. coli Polar Lipid Extract
表单
chloroform solution
包装
pkg of 2 × 20 mL (100600C-1g)
pkg of 1 × 4 mL (100600C-100mg)
pkg of 5 × 4 mL (100600C-500mg)
制造商/商品名称
Avanti Research™ - A Croda Brand
浓度
25 mg/mL (100600C-100mg)
25 mg/mL (100600C-1g)
25 mg/mL (100600C-500mg)
脂质类型
lipid extracts
运输
dry ice
储存温度
−20°C
一般描述
This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated. Polar lipid extract is the total lipid extract precipitated with acetone and then extracted with diethyl ether.
应用
E. coli Extract Polar is suitable to prepare small unilamellar vesicles, to mimic the surface of the E. coli inner membrane.
E. coli extract polar has been for the preparation of liposomes.
E. coli Extract Polar has been used:
- in unilamellar liposomes preparation for kinetic studies
- in the reconstitution of ATP-binding cassette (ABC) transporter for electron paramagnetic resonance spectroscopy studies
- in the reconstitution of molybdate transport protein (MolBC) for continuous wave-electron paramagnetic resonance studies
生化/生理作用
E.coli Polar lipid extracts comprise phosphatidylethanolamine as major lipid component. The minor components include the phosphatidylglycerol (PG) and cardiolipin. The leaflets of cytoplasmic membrane and inner leaflet of outer membrane are the source of E. coli lipid extract. It is used for reconstituting membrane proteins and forms bilayer by vesicle fusion.
包装
30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (100600C-1g)
5 mL Clear Glass Sealed Ampule (100600C-100mg)
5 mL Clear Glass Sealed Ampule (100600C-500mg)
法律信息
Avanti Research is a trademark of Avanti Polar Lipids, LLC
警示用语：
Danger
危险分类
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
靶器官
Central nervous system, Liver,Kidney
储存分类代码
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
易制毒化学品（2类）
危险化学品
An inducible reconstitution system for the real-time kinetic analysis of protease activity and inhibition inside the membrane
Baker RP and Urban S
Methods in Enzymology, 584, 229-253 (2017)
The
Enzymology Primer for Recombinant DNA Technology (2017)
Small substrate transport and mechanism of a molybdate ATP binding cassette transporter in a lipid environment
Rice AJ, et al.
The Journal of biological chemistry, 289(21), 15005-15013 (2014)
Emily Peterson et al.
Biochimica et biophysica acta, 1808(2), 516-521 (2010-10-26)
Amantadine-sensitive proton uptake by liposomes is currently the preferred method of demonstrating M2 functionality after reconstitution, to validate structural determination with techniques such as solid-state NMR. With strong driving forces (two decades each of both [K(+)] gradient-induced membrane potential and
Kohki Fujikawa et al.
ACS chemical biology, 13(9), 2719-2727 (2018-08-02)
MPIase is the first known glycolipid that is essential for membrane protein integration in the inner membrane of E. coli. Since the amount of natural MPIase available for analysis is limited and it contains structural heterogeneity, precisely designed synthetic derivatives
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