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关于此项目
经验公式（希尔记法）:
C32H58N2O7S
化学文摘社编号:
分子量:
614.88
MDL number:
UNSPSC Code:
12161902
NACRES:
NA.25
产品名称
CHAPS, Avanti Research™ - A Croda Brand
SMILES string
C[C@@]12C(CC[C@@H]2[C@@H](CCC(NCCC[N+](C)(CCCS(=O)([O-])=O)C)=O)C)C3[C@H](O)C[C@]4([H])C[C@H](O)CC[C@]4(C)C3C[C@@H]1O
InChI
1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
InChI key
UMCMPZBLKLEWAF-BCTGSCMUSA-N
assay
>99% (TLC)
form
powder
mol wt
614.88 g/mol
packaging
pkg of 1 × 1 g (850500P-1g)
manufacturer/tradename
Avanti Research™ - A Croda Brand
technique(s)
protein quantification: suitable
shipped in
dry ice
storage temp.
−20°C
Application
CHAPS适用于制备双细胞，并研究脂质体溶解和转运蛋白复溶的过程。
General description
CHAPS是用于膜生物化学的非变性两性离子去污剂。可用于溶解膜蛋白和破坏蛋白-蛋白各种相互作用。CHAPS的小胶束分子量（6,150；聚集数=10）和高临界胶束浓度（6-10 mM）使其可以通过透析从样品中除去。也适用于等电聚焦和二维电泳的蛋白增溶。CHAPS通常用于非变性（无尿素）IEF，并已显示出某些亚细胞制剂和植物蛋白具有出色的分离度。IEF凝胶通常使用2-4%（w/v）的浓度。
Packaging
20 mL透明玻璃螺旋盖小瓶（850500P-1g）
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Functional reconstitution of the nicotinic acetylcholine receptor by CHAPS dialysis depends on the concentrations of salt, lipid, and protein
Schuerholz T, et al.
Biochemistry, 31(21), 5067-5077 (1992)
Liposome solubilization and membrane protein reconstitution using Chaps and Chapso
Cladera J, et al.
European Journal of Biochemistry, 243(3), 798-804 (1997)
Bilayer membrane modulation of membrane type 1 matrix metalloproteinase (MT1-MMP) structure and proteolytic activity
Cerofolini L, et al.
Scientific reports, 6, 29511-29511 (2016)
Stuart J Cordwell
Methods in molecular biology (Clifton, N.J.), 424, 139-146 (2008-03-29)
Reproducible techniques for the prefractionation of proteins prior to two-dimensional gel electrophoresis (2-DE) are essential for increasing the number of unique proteins that can be identified and assayed following biological experimentation. A simple and robust technique for separating highly soluble
Erik F Kot et al.
Langmuir : the ACS journal of surfaces and colloids, 34(11), 3426-3437 (2018-02-28)
Isotropic phospholipid bicelles are one of the most prospective membrane mimetics for the structural studies of membrane proteins in solution. Recent works provided an almost full set of data regarding the properties of isotropic bicelles; however, one major aspect of
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