产品名称
SMC ®人IL-17A高灵敏度试剂盒, Used for ultrasensitive quantification of Human IL-17A in human serum and plasma
manufacturer/tradename
SMC®
product line
SMC®
usage
kit sufficient for 96 wells
species reactivity
human
technique(s)
single molecule counting: suitable
input
plasma (K2 EDTA)
serum
UniProt accession no.
compatibility
for use with FemtoQuest™ and SMCxPRO® instruments
shipped in
wet ice
storage temp.
2-8°C
Biochem/physiol Actions
Disclaimer
General description
测定方案与现有的夹心ELISA方法相似,但有两个主要区别:
1)洗脱缓冲液破坏夹心结构,分离标记的检测抗体以进行定量。
2)Erenna 和SMCxPRO® 免疫测定系统使用单分子计数(SMC®)技术对分析物进行检测。
Legal Information
Packaging
Analysis Note
- Sensitivity: LLOQ 70 fg/mL* (SMCxPRO® instrument
- Standard Curve Range: 20-60,000 fg/mL
- Intra-assay CV: <15%
- Inter-assay CV: <20%
- Linearity: Serum: 106% Plasma: 93%
- Recovery: Serum: 92% Plasma: 103%
* Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
Application
Features and Benefits
- Unmatched Detection Capability: Utilizing SMC® technology, this assay offers superior performance in quantifying IL-17A in human serum and plasma, ensuring precise and reliable measurements.
- Advance Autoimmune & Inflammatory Disease Research: IL-17A is a key cytokine in Th17-mediated immunity. This assay supports studies in psoriasis, rheumatoid arthritis, IBD, and other chronic inflammatory conditions.
- Enable Early Biomarker Discovery: High sensitivity allows detection of subclinical cytokine shifts, supporting early-stage biomarker identification and therapeutic target validation.
- Improve Data Quality & Confidence: Low background and high signal-to-noise ratio ensure robust, publication-ready data.
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
法规信息
实验方案
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
相关内容
蛋白定量方法、试剂和免疫测定技术可准确测定各类样品中的蛋白浓度。
Read an automated protocol for SMC® immunoassays on the AAW™ automated assay workstation and compare manual vs automated runs.
Learn how immunoassays are used in cosmetics and personal care research to discover potential harmful effects of cosmetics products, and assess markers of inflammation, sensitization, aging, and tissue regeneration among others.
Use the power of high sensitivity immunology assays with SMC® technology to precisely characterize low-abundant cytokines like those from Th1/Th2 and Th17 cells.
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