产品名称
SMC® 人TNFα 高灵敏度试剂盒, Used for ultrasensitive quantification of Human TNFα in human serum and plasma
manufacturer/tradename
SMC®
assay range
sensitivity: 0.20 pg/mL
(lower limit of quantification)
inter-assay cv: <20%
intra-assay cv: <15%
standard curve range: 0.05-50 pg/mL
product line
SMC®
usage
kit sufficient for 96 wells
species reactivity
human
technique(s)
single molecule counting: suitable
input
plasma (K2 EDTA)
serum
UniProt accession no.
compatibility
for use with FemtoQuest™ and SMCxPRO® instruments
shipped in
wet ice
storage temp.
2-8°C
Biochem/physiol Actions
Disclaimer
General description
测定方案与现有的夹心ELISA方法相似，但有两个主要区别：
1）洗脱缓冲液破坏夹心结构，分离标记的检测抗体以进行定量。
2）Erenna 和SMCxPRO® 免疫测定系统使用单分子计数（SMC®）技术对分析物进行检测。
Legal Information
Analysis Note
- Sensitivity: LLOQ 200 fg/mL* (SMCxPRO® Instrument
- Standard Curve Range: 50-50,000 fg/mL
- Intra-assay CV: <15%
- Inter-assay CV: <20%
- Linearity: Serum: 113% Plasma: 105%
- Recovery: Serum: 95% Plasma: 83%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
Application
Features and Benefits
- Ultra-Sensitive Detection: Utilizing SMC®technology, this assay detects TNFα at femtogram-level concentrations, enabling precise quantification in human plasma and serum—even in low-expression conditions.
- Familiar Workflow, Elevated Performance: Follows a standard sandwich immunoassay protocol with a unique elution step and digital readout, delivering high signal-to-noise ratios and low background interference.
- Expert Scientific Support: Backed by Field Application Scientists and technical support teams experienced in academic, pharma, CRO, and regulated lab environments.
- Automation-Ready Workflow: Compatible with liquid handling systems for hands-free operation, reducing variability and increasing throughput.
Packaging
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
法规信息
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