选择尺寸
关于此项目
technique(s)
single molecule counting: suitable
input
plasma (K2 EDTA)
serum
UniProt accession no.
compatibility
for use with FemtoQuest™ and SMCxPRO® instruments
shipped in
wet ice
storage temp.
2-8°C
product line
SMC®
usage
kit sufficient for 96 wells
species reactivity
human
General description
测定方案与现有的夹心ELISA方法相似,但有两个主要区别:
1)洗脱缓冲液破坏夹心结构,分离标记的检测抗体以进行定量。
2)Erenna 和SMCxPRO® 免疫测定系统使用单分子计数(SMC®)技术对分析物进行检测。
Application
Features and Benefits
- Detect the Undectable: Detects IL-17F at concentrations as low as 200 fg/mL, enabling precise quantification of low-abundance cytokines in human plasma and serum.
- Wide Dynamic Range: Standard curve spans 100-100,000 fg/mL, supporting both basal and elevated IL-17F levels in diverse sample types.
- Advance Th17 Pathway Research: IL-17F is a critical cytokine in autoimmune and inflammatory diseases such as asthma, psoriasis, and IBD. This assay supports mechanistic studies and therapeutic development.
- Enable Early Biomarker Discovery: Detect subclinical cytokine shifts with high sensitivity, ideal for identifying early disease markers and monitoring immune responses.
Packaging
Analysis Note
- Sensitivity: LLOQ 200 fg/mL* (SMCxPRO® Instrument)
- Standard Curve Range: 100-100,000 fg/mL
- Intra-assay CV: <15%
- Inter-assay CV: <20%
- Linearity: Serum: 99% Plasma: 101%
- Recovery: Serum: 94% Plasma: 105%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
Other Notes
Legal Information
Disclaimer
signalword
Danger
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B
法规信息
相关内容
Read an automated protocol for SMC® immunoassays on the AAW™ automated assay workstation and compare manual vs automated runs.
Learn how immunoassays are used in cosmetics and personal care research to discover potential harmful effects of cosmetics products, and assess markers of inflammation, sensitization, aging, and tissue regeneration among others.
Use the power of high sensitivity immunology assays with SMC® technology to precisely characterize low-abundant cytokines like those from Th1/Th2 and Th17 cells.
在 AAW™ 自动移液工作站上读取 SMC®免疫测定的自动协议,并比较手动与自动运行。
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