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安全信息

03-0176-00

Millipore

SMC® Human IL-10 High Sensitivity Immunoassay Kit

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种属反应性

rat, mouse, feline, canine, nonhuman primates

制造商/商品名称

SMC®

assay range

sensitivity: 0.10 pg/mL
(Lower Limit of Quantification:)

inter-assay cv: <20%
intra-assay cv: <15%
(Intra-assay n=20; Inter-assay n=5)

技术

single molecule counting: suitable

储存温度

2-8°C

一般描述

The SMC® Human IFN-b1 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.

特异性

Species Reactivity
Although IL-10 was detectable in non-human primate, mouse, rat, canine, and feline plasma, the assay has not been validated for these species.

Accuracy:
  • Minimum of 5 serum samples spiked with average recovery of 87% with a range of 84-93%
  • Minimum of 5 plasma samples spiked with average recovery of 90% with a range of 82-97%

应用

The sensitivity of the Single Molecule Counting (SMC®) technology gives the SMC® Human IL-10 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-10 in human serum and plasma.

This new streamline kit has been improved with the following features including new including new improvements over the legacy IL-10 kit, #03-0056-00:
  • Improved LLOQ--0.10 pg/mL in the new streamlined kit while the legacy kit is 0.39 pg/mL
  • Adjusted standard curve--changed to 0.1 – 100 pg/mL from 0.17 – 400 pg/mL
  • Improved options for sample prep and incubation
  • Lyophilization of the standard provides the kit at one storage condition
  • Removal of a plate transfer step
  • Increased reagent volumes to accommodate automation
  • Validated to run on both SMCxPRO® and Erenna


Research Category:
Inflammation & Immunology

Research Sub-Category:
  • Inflammatory Disease
  • Immune Response
  • Autoimmune disease

包装

96 well assay

组分

  • Assay Buffer
  • IL-10 Coated Beads
  • Standard Diluent
  • IL-10 Detection Antibody
  • IL-10 Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B

法律信息

SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形图

Skull and crossbonesHealth hazard

警示用语:

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B

储存分类代码

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

法规信息

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