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Merck
CN

03-0195-00

SMC® Human IL-2 High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IL-2 in human serum and plasma

别名:

IL-2 Single Molecule Counting kit, IL-2 immunoassay kit, IL-2 ultrasensitive immunoassay, interleukin-2 immunoassay

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UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000
Species reactivity:
human
Technique(s):
single molecule counting: suitable
Usage:
 kit sufficient for 96 wells
Compatibility:
for use with FemtoQuest™ and SMCxPRO® instruments

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description

for protein analysis

technique(s)

single molecule counting: suitable

input

plasma (K2 EDTA)
serum

UniProt accession no.

P60568

compatibility

for use with FemtoQuest™ and SMCxPRO® instruments

shipped in

wet ice

storage temp.

2-8°C

product line

SMC®

usage

 kit sufficient for 96 wells

feature

accuracy Minimum of 5 plasma samples spiked with average recovery of 83% with a range of 78-87%, accuracy Minimum of 5 serum samples spiked with average recovery of 91% with a range of 79-99%

species reactivity

human

相关类别

General description

Interleukin-2 (IL-2) is a crucial cytokine in the immune system, primarily produced by T cells. It plays a vital role in the proliferation, differentiation, and activation of immune cells, particularly T lymphocytes and natural killer (NK) cells. IL-2 is essential for maintaining immune homeostasis and is a key factor in the body′s response to infections and tumors.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Features and Benefits

  • Breakthrough-Level Sensitivity For Translational Research: Supports early-stage biomarker identification and immune profiling with unmatched precision.
  • Support Autoimmune and Inflammatory Studies: Quantify IL-2 in models of autoimmunity, graft rejection, or chronic inflammation.
  • Illuminate Early Immune Activation: IL-2 is a key marker of T-cell activation; this assay helps detect it even at the earliest stages of immune response.
  • High Precision and Reproducibility: Verified across multiple operators and days, ensuring consistent, reliable results

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 90 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 20-80,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 108% Plasma: 104%
  • Recovery: Serum: 91% Plasma 83%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although IL-2 was detectable in non-human primate, canine, and feline samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B

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