Calpeptin

A cell-permeable calpain inhibitor (ID₅₀ = 52 nM for calpain-1; ID₅₀ = 34 nM for calpain-2; ID₅₀ = 138 nM for papain). Inhibits the Ca²⁺-stimulated cleavage of p35 to p25 by calpain. Modulates the processing of β-amyloid precursor protein to β-amyloid (Aβ). Promotes neurite elongation in differentiating PC12 cells. Prevents Ca²⁺-ionophore-induced degradation of actin binding protein and P₂₃₅ in platelets. Inhibits myosin light chain phosphorylation in platelets stimulated by collagen, ionomycin, or thrombin. Inhibits the growth of estrogen receptor positive breast cancer cells.

A cell-permeable calpain inhibitor. Inactivates calpain I (ID₅₀ = 52 nM), calpain II (ID₅₀ = 34 nM), and papain (ID₅₀ = 138 nM). Promotes neurite elongation in differentiating PC12 cells. Prevents Ca²⁺-ionophore induced degradation of actin binding protein and P₂₃₅ in platelets. Inhibits myosin light chain phosphorylation in platelets stimulated by collagen, ionomycin, or thrombin. Inhibits the growth of estrogen receptor positive breast cancer cells.

Cell permeable: yes

ID50 = 52 nM for calpain-1; 34 nM for calpain-2; 138 nM for papain

Product does not compete with ATP.

Reversible: no

Packaged under inert gas

Toxicity: Standard Handling (A)

Z-Leu-Nle-CHO

Dissolve in a minimal amount of DMF or DMSO then add H₂O, buffer or medium just prior to use.

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Saito, K., and Nixon, R.A. 1993. Neurochem. Res. 18, 231.
Tsujinaka, T., et al. 1988. Biochem. Biophys. Res. Commun.153, 1201.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany