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Merck
CN

04-001

抗JAK2抗体，克隆8E10.2

clone 8E10.2, Upstate®, from mouse

别名:

Janus kinase 2, Tyrosine kinase

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.43
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
8E10.2, monoclonal
Application:
IHC, WB
Citations:
10

主要文件

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biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

8E10.2, monoclonal

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_004963

UniProt accession no.

O60674

shipped in

ambient

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... JAK2(3717)
mouse ... Jak2(16452)
rat ... Jak2(24514)

General description

130kda
JAK2（Janus 激酶 2）属于新兴的非受体 Janus 酪氨酸激酶家族，可调节淋巴造血系统中活化细胞因子受体下游的一系列细胞功能。免疫刺激物（例如干扰素和细胞因子）诱导 Stat 转录因子募集到与细胞因子受体相关的 JAK2 上。然后，JAK2 使近端 Stat 因子磷酸化，随后将其二聚化并转运至细胞核，并与靶基因启动子上游的 CIS 元件结合，从而调节转录。经典的 JAK/Stat 途径通过刺激病原体的增殖、分化、存活和宿主抗性，是维持正常免疫系统不可或缺的一部分。改变 JAK/Stat 信号传导以减少细胞因子诱导的促炎反应代表了抗炎治疗的有吸引力的靶标。

Immunogen

KLH 偶联的线性肽。

Application

蛋白质印迹分析：0.5-1 μg/mL 的该批次用于 L6 细胞裂解物中的 Jak2。

免疫组化（石蜡）：
先前批次的该抗体大量用于表位修复的免疫组化（石蜡）染色：人癌症阵列。
抗 JAK2 抗体，克隆 8E10.2 可检测 JAK2 水平，&经发布 & 验证可用于 WB、IH（P）。
研究子类别
免疫信号通路
研究类别
炎症&免疫学

Biochem/physiol Actions

该抗体识别 Jak2。

Physical form

含 0.1 M Tris-甘氨酸（pH7.4），150 mM NaCl 和 0.05％ 叠氮化钠的缓冲液中的纯化小鼠单克隆 IgG1κ
形式：纯化的
蛋白 G 纯化

Preparation Note

自收到之日起，在 2-8°C 条下可稳定保存 1 年。

Analysis Note

对照
L6 细胞裂解液
通过蛋白质印迹对 L6 细胞裂解物进行了常规评估。

Other Notes

浓度：请参考批次特异性浓缩物的分析证书。

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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Pasquale Esposito et al.
Brain, behavior, & immunity - health, 26, 100543-100543 (2022-11-09)
Exposure to stressors during puberty can cause enduring effects on brain functioning and behaviours related to neurodegeneration. However, the mechanisms underlying these effects remain unclear. The gut microbiome is a complex and dynamic system that could serve as a possible
Virally infected and matured human dendritic cells activate natural killer cells via cooperative activity of plasma membrane-bound TNF and IL-15.
Vujanovic L, Szymkowski DE, Alber S, Watkins SC, Vujanovic NL, Butterfield LH
Blood null
Lili Guo et al.
Scientific reports, 6, 32093-32093 (2016-08-27)
Individuals with poor postnatal growth are at risk for cardiovascular and metabolic problems as adults. Here we show that disruption of the molecular scaffold Kinase Suppressor of Ras 2 (KSR2) causes selective inhibition of hepatic GH signaling in neonatal mice
Y Senis et al.
Molecular and cellular biology, 19(11), 7436-7446 (1999-10-19)
The fps/fes proto-oncogene encodes a cytoplasmic protein-tyrosine kinase that is functionally implicated in the survival and terminal differentiation of myeloid progenitors and in signaling from several members of the cytokine receptor superfamily. To gain further insight into the physiological function
Frauke Ringel et al.
Acta haematologica, 132(1), 75-86 (2014-02-08)
Janus kinases are critical components of signaling pathways that regulate hematopoiesis. Mutations of the non-receptor tyrosine kinase JAK2 are found in many BCR-ABL-negative myeloproliferative neoplasms. Preclinical results support that JAK2 inhibitors could show efficacy in treating chronic myeloproliferative neoplasms. JAK2
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相关内容

JAK/STAT Signaling Research Focus

The Janus kinase (JAK)/signal transducers and activators of transcription (STAT) signaling pathway plays an important role in cell proliferation, cell differentiation, cell migration, and cell death. It is the principal signaling mechanism for a variety of cytokines and growth factors. Constitutive activation or dysregulation of JAK/STAT signaling can result in inflammatory disease, erythrocytosis, gigantism, and leukemia.

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