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Merck
CN

04-085

Anti-Ago family Antibody

Upstate®, from mouse

别名:

argonaute family, piwi

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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产品名称

Anti-Ago family Antibody, Upstate®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG1

NCBI accession no.

NM_012154.2
NM_012199.2

UniProt accession no.

Q9UKV8
Q9UL18

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... AGO1(26523)

Analysis Note

Routinely evaluated by immunoblot.

Application

Suggested dilution:
Western Blot: 1:500
Research Category
Protein Trafficking
Research Sub Category
RNA Metabolism & Binding Proteins

RNA Binding Protein (RBP)
Use Anti-Ago family Antibody (Mouse Monoclonal Antibody) validated in WB to detect Ago family also known as argonaute family PIWI.

Biochem/physiol Actions

Ago family proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

~100 kDa

Immunogen

Synthetic peptide PKIDVYHYEVDIKPDKCPRRVNREVV

Physical form

100 μg of Protein A purified mouse monoclonal IgG1κ in 100μl of 1X PBS, pH 7.0, 0.1% Azide.
Format: Purified
Protein A purified

Preparation Note

2 years at -20°C from date of shipment

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Interactions between the RNA interference effector protein Ago1 and 14-3-3 proteins: consequences for cell cycle progression
Stoica, Cezar, et al
The Journal of Biological Chemistry, 281, 37646-37651 (2006)
Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi
Yigit, Erbay, et al
Cell, 127, 747-757 (2006)
Wibke J Meyer et al.
PLoS genetics, 2(8), e134-e134 (2006-08-29)
Argonaute proteins are essential components of the molecular machinery that drives RNA silencing. In Drosophila, different members of the Argonaute family of proteins have been assigned to distinct RNA silencing pathways. While Ago1 is required for microRNA function, Ago2 is
Differential remodeling of mono- and trimethylated H3K27 during porcine embryo development.
Park KE, Magnani L, Cabot RA
Molecular Reproduction and Development null

相关内容

RNA-Binding Protein Immunoprecipitation

All eukaryotic organisms require tight regulation of gene expression for complex processes such as development, differentiation, cell specification, and responses to environmental stimuli. Many genes are regulated post-transcriptionally, in addition to transcriptional mechanisms of gene regulation. RNA-binding proteins (RBPs) are essential for post-transcriptional gene regulation, linking transcription and translation in many processes including transcription, splicing, export, rate of translation and turnover. In all of these events, RBPs coordinate regulation of the amount of protein produced from mRNA transcripts.

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