04-1525
Anti-TAFII250 Antibody, clone 6B3
clone 6B3, from mouse
别名:
Cell cycle gene 1 protein, TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa, TAF1 RNA polymerase II, TATA box binding protein-associated factor, 250kDa, TATA box binding protein (TBP)-associated factor, RNA polymerase II,
产品名称
Anti-TAFII250 Antibody, clone 6B3, clone 6B3, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6B3, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TAF1(6872)
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected TAFII250 on 10 µg of HeLa cell lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected TAFII250 on 10 µg of HeLa cell lysate.
Application
Anti-TAFII250 Antibody, clone 6B3 is a Mouse Monoclonal Antibody for detection of TAFII250 also known as Cell cycle gene 1 protein & has been validated in WB, ICC, IHC & IP.
Immunoprecipitation
Cited by external research group using a representative lot. See Hilton, H.L., et. al (2005) Mol. Cell. Bio. 25(10):4321–4332.
Cited by external research group using a representative lot. See Hilton, H.L., et. al (2005) Mol. Cell. Bio. 25(10):4321–4332.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Biochem/physiol Actions
This antibody recognizes TAFII250.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Calculated molecular weight is 212 kDa However protein is usually observed at 250 kDa band.
TATA-binding protein associated factor 250 (TAFII250) is the largest subunit of the transcription factor TFIID. Studies have shown that TAFII250 has serine/threonine domains that can autophosphorylate and transphosphorylate the large subunit of TFIIF. More recent studies have shown that TAFII250 is also a histone acetyltransferase, suggesting that transcriptional co-activators may also have a role in targeting acetylation activity to promoters.
Immunogen
Epitope: Unknown
Human TAFII250
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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