04-281
Anti-phospho-EGFR (Thr669) Antibody, clone 5F10
clone 5F10, Upstate®, from mouse
别名:
ERBB, ERBB1, epidermal growth factor receptor
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
5F10, monoclonal
Species reactivity:
human
Application:
WB
Citations:
7
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5F10, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pThr669)
Gene Information
human ... EGFR(1956)
General description
180 kDa
Immunogen
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 669 in human EGFR.
Application
Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 detects level of phospho-EGFR (Thr669) & has been published & validated for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Biochem/physiol Actions
Recognizes EGFR when phosphorylated at Threonine 669.
Physical form
100 µg of mouse monoclonal IgG1 liquid in 0.01 mg/ml PBS containing 0.09% sodium azide, PEG, and sucrose with 50 % glycerol.
Format: Purified
Thiophilic adsoption and size exclusion chromatography
Preparation Note
6 months at -20°C from date of shipment
Analysis Note
Control
Includes PMA and pervanadate treated A431 cell lysate as a positive control
Includes PMA and pervanadate treated A431 cell lysate as a positive control
Routinely evaluated by immunoblot.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
G J Heisermann et al.
The Journal of biological chemistry, 263(26), 13152-13158 (1988-09-15)
The epidermal growth factor (EGF) receptor is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/phospholipid-dependent enzyme) mediated phosphorylation at identified sites. The EGF receptor contains additional phosphorylation sites including a prominent phosphothreonine and several phosphoserines which
Mutational removal of the Thr669 and Ser671 phosphorylation sites alters substrate specificity and ligand-induced internalization of the epidermal growth factor receptor
Heisermann, G J, et al
The Journal of Biological Chemistry, 265, 12820-12827 (1990)
Cisplatin induces PKB/Akt activation and p38(MAPK) phosphorylation of the EGF receptor
Winograd-Katz, S E and Levitzki, A
Oncogene, 25, 7381-7390 (2006)