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Merck
CN

04-300

Anti-phospho-Insulin Receptor (Tyr 1322) Antibody, clone 21G12

clone 21g12, Upstate®, from mouse

别名:

INSR

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
21g12, monoclonal
Species reactivity:
canine, human, mouse
Application:
ELISA, WB
Citations:
12
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biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

21g12, monoclonal

species reactivity

canine, human, mouse

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pTyr1322)

Gene Information

human ... INSR(3643)

General description

97 kDa

Immunogen

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyrosine 1322 on phosphorylated insulin receptor

Application

Anti-phospho-Insulin Receptor Antibody, clone 21G12 is an antibody against phospho-Insulin Receptor (Tyr 1322) for use in ELISA & WB.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Biochem/physiol Actions

Recognizes phosphorylated domain of Insulin receptor on Tyrosine 1322.

Physical form

Format: Purified
Lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose.
Subsequent thiophilic adsorption and size exclusion chromatography

Preparation Note

2 years at -20°C from date of shipment. Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Includes pervanadate treated HEK293 lysate as a positive control.
Routinely evaluated by immunoblot.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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存储类别

10 - Combustible liquids



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Francesca Fiory et al.
Molecular and cellular biology, 25(24), 10803-10814 (2005-11-30)
In L6 myoblasts, insulin receptors with deletion of the C-terminal 43 amino acids (IR(Delta43)) exhibited normal autophosphorylation and IRS-1/2 tyrosine phosphorylation. The L6 cells expressing IR(Delta43) (L6(IRDelta43)) also showed no insulin effect on glucose uptake and glycogen synthase, accompanied by
Qiu-yan Wang et al.
The FEBS journal, 274(2), 526-538 (2007-01-19)
Two H7721 human hepatocarcinoma cell lines showing moderate and high expression of alpha1,3-fucosyltransferase (FucT)-VII cDNA were established and designated FucTVII-M and FucTVII-H, respectively. In alpha1,3-FucT-VII-transfected cells, expression of insulin receptor (InR) alpha- and beta subunits and epidermal growth factor receptor
Template to improve glycemic control without reducing adiposity or dietary fat.
Krishnapuram, R; Dhurandhar, EJ; Dubuisson, O; Kirk-Ballard, H; Bajpeyi, S; Butte et al.
American Journal of Physiology. Endocrinology and Metabolism null



全球贸易项目编号

货号GTIN
04-30004053252365065
GE17-0756-0404061837620676