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04-721

抗Dicer1抗体，克隆5D12.2

clone 5D12.2, Upstate®, from mouse

别名:

DCR1, Dicer, HERNA, KIAA0928

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
5D12.2, monoclonal
Application:
WB
Citations:
5
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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

5D12.2, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μL

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_085124.2

UniProt accession no.

Q9UPY3

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... DICER1(23405)

General description

220kda
RNA 干扰（RNAi）是转录后基因沉默的机制，导致基因活性丧失。当长的 dsRNA 被 Dicer1（dsRNA 特异性核酸内切酶的 RNase III 家族）酶切割成 21-23 个核苷酸的 siRNA（小干扰 RNA）时，RNAi 被激活。这些 siRNA 与蛋白质结合形成 RISC（RNA 诱导的沉默复合体），当双链 siRNA 解开成单链 siRNA 时，RISC 被激活。激活的 RISC 识别并结合 mRNA 中的互补序列。mRNA 被切割，总体效果是抑制蛋白质表达和基因活性的丧失。Dicer1 与 Argonaute 蛋白质家族（Ago1 和 Ago2）共享相同的结构域（PIWI 和 PAZ 结构域），这些区域为 RNA 诱导的沉默复合物（RISC）提供核酸内切酶活性。

Immunogen

对应于人 Dicer1 氨基酸 378-385 的 KLH 偶联的合成肽

Application

抗 Dicer1 抗体，克隆 5D12.2 是一种用于检测 Dicer1（也称为 DCR1、Dicer、HERNA、KIAA0928）的小鼠单克隆抗体，&已在 WB 中得到验证。
研究子类别
RNA 代谢&结合蛋白

RNA 结合蛋白（RBP）
研究类别
表观遗传学&核功能
蛋白质印迹

Biochem/physiol Actions

人 Dicer1

Physical form

形式：纯化的
蛋白质 G 色谱

Preparation Note

自收到之日起，在 2-8° 以未稀释的等分试样可保存 6 个月。

Analysis Note

通过蛋白质印迹对 HeLa 核提取物的裂解液进行了常规评估。

Other Notes

请参考特定批次的数据表。

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

存储类别

12 - Non Combustible Liquids

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货号GTIN
04-721-25UL04054839350146